LOCUS BBa_J100045 2823 bp DNA linear 22-Dec-2024 DEFINITION LuxI/GFP with 4 clause 2-SAT problem ACCESSION BBa_J100045 VERSION BBa_J100045.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 terminator 1..129 /label=BBa_B0015 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 CDS 219..922 /label=BBa_J100044 RBS 931..945 /label=RBS-1\Strong RBS 938..942 /label=RBS misc_feature 931..945 /label=BBa_B0030 RBS 931..945 /label=BBa_B0030 CDS 952..1671 /label=GFP protein CDS 952..1671 /label=BBa_E0040 CDS 1680..1767 /label=end of LacI coding region (inactive) misc_feature 1680..1879 /label=BBa_R0010 misc_feature 1768..1805 /label=CAP binding site promoter 1816..1821 /label=-35 promoter 1840..1845 /label=-10 misc_feature 1845..1879 /label=LacI binding site misc_feature 1852..1852 /label=start promoter 1680..1879 /label=BBa_R0010 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 misc_feature 1906..1907 /label=prefix misc_feature 2079..2079 /label=T misc_feature 2397..2397 /label=A CDS 1906..2655 /label=luxR misc_feature 1906..2661 /label=BBa_C0062 misc_feature 2662..2686 /label=Help:Barcodes CDS 1906..2661 /label=BBa_C0062 stem_loop 2706..2749 /label=stem_loop misc_feature 2695..2774 /label=BBa_B0010 terminator 2695..2774 /label=BBa_B0010 stem_loop 2790..2809 /label=T7 TE misc_feature 2816..2816 /label=stop polyA_site 2810..2823 /label=polya misc_feature 2783..2823 /label=BBa_B0012 terminator 2783..2823 /label=BBa_B0012 terminator 2695..2823 /label=BBa_B0015 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgcg ccactgctag 241 tgtaataatt ctagtgcggt cgtaataatt ccactgcggt cgtaataatt ctagtgccat 301 cgtaataatt gggcgcgccg caattgcaag cggtcgtgca accattatga ttaaaaaaag 361 cgattttctg gccattccga gcgaagaata taaaggtatt ctgagcctgc gctatcaggt 421 ttttaaacag cgtctggaat gggatctggt ggttgaaaat aatctggaaa gtgatgaata 481 tgataatagc aatgccgaat atatttatgc ctgtgatgat accgaaaatg ttagcggttg 541 ttggcgtctg ctgccgacca ccggtgatta tatgctgaaa agcgtttttc cggaactgct 601 gggtcagcag agcgcaccga aagatccgaa tattgttgaa ctgagccgtt ttgccgtggg 661 taaaaatagc agcaaaatta ataatagcgc cagcgaaatt accatgaaac tgtttgaagc 721 catttataaa catgccgtta gccagggtat taccgaatat gttaccgtta ccagcaccgc 781 aattgaacgt tttctgaaac gtattaaagt gccgtgtcat cgtattggcg ataaagaaat 841 tcatgttctg ggcgatacca aaagcgttgt tctgagcatg ccgattaatg aacagtttaa 901 aaaagccgtg ctgaactaat aatactagag attaaagagg agaaatacta gatgcgtaaa 961 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 1021 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 1081 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 1141 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 1201 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 1261 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 1321 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 1381 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt 1441 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa 1501 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca 1561 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt 1621 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc 1681 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 1741 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 1801 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 1861 cggataacaa tttcacacat actagagaaa gaggagaaat actagatgaa aaacataaat 1921 gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt 1981 aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc 2041 atttatcctc attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa 2101 tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt agattattct 2161 aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa 2221 tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct 2281 attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac 2341 tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta 2401 gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa 2461 agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa 2521 atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc 2581 aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc 2641 ccatacttta aaaattaata acactgatag tgctagtgta gatcactact agagccaggc 2701 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2761 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2821 ata //