##gff-version 3
##sequence-region BBa_J100045 1 2823
BBa_J100045	.	CDS	1906	2661	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J100045	.	engineered_region	1906	1907	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J100045	.	engineered_tag	2662	2686	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J100045	.	point_mutation	2397	2397	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J100045	.	CDS	1906	2655	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J100045	.	engineered_region	1906	2661	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J100045	.	point_mutation	2079	2079	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J100045	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J100045	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J100045	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J100045	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J100045	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J100045	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J100045	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100045	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100045	.	ribosome_entry_site	931	945	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J100045	.	ribosome_entry_site	931	945	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J100045	.	engineered_region	931	945	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J100045	.	ribosome_entry_site	938	942	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J100045	.	ribosome_entry_site	1888	1899	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J100045	.	conserved_region	1892	1895	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J100045	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100045	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100045	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100045	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100045	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100045	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100045	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100045	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100045	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100045	.	CDS	952	1671	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J100045	.	CDS	952	1671	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100045	.	terminator	2695	2823	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J100045	.	terminator	2695	2774	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J100045	.	engineered_region	2695	2774	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100045	.	stem_loop	2706	2749	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100045	.	terminator	2783	2823	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J100045	.	stem_loop	2790	2809	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100045	.	polyA_site	2810	2823	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100045	.	stop_codon	2816	2816	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100045	.	engineered_region	2783	2823	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100045	.	promoter	1680	1879	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J100045	.	engineered_region	1680	1879	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J100045	.	CDS	1680	1767	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J100045	.	promoter	1840	1845	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J100045	.	non_covalent_binding_site	1845	1879	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J100045	.	promoter	1816	1821	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J100045	.	non_covalent_binding_site	1768	1805	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J100045	.	start_codon	1852	1852	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J100045	.	CDS	219	922	.	+	0	ID=BBa_J100044;Name=BBa_J100044
>BBa_J100045
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcgccactgc
tagtgtaataattctagtgcggtcgtaataattccactgcggtcgtaataattctagtgccatcgtaataattgggcgc
gccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaag
gtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtga
tgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctg
ccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaata
ttgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaact
gtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgt
tttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttg
ttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaatac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctt
tacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggag
aaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatg
atattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattc
tatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaata
aaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaa
ataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggc
taacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgt
atgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacg
atttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattagg
ttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatt
tctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata