LOCUS       BBa_J100050              938 bp    DNA     linear       29-May-2024
DEFINITION  pBad+BD18+RFP_withoutATG
ACCESSION   BBa_J100050
VERSION     BBa_J100050.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             157..165
                     /label=leader RBS
     misc_feature    171..173
                     /label=Start
     misc_feature    222..224
                     /label=Stop
     misc_feature    224..226
                     /label=Start
     RBS             209..217
                     /label=GOI RBS
     misc_feature    139..226
                     /label=BBa_J119024
     CDS             233..907
                     /label=mrfp1
     misc_feature    914..938
                     /label=Help:Barcodes
     CDS             233..913
                     /label=BBa_E1010
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggg gcccaagttc acttaaaaag gagatcaaca atgaaagcaa
      181 ttttcgtact gaaacatctt aatcatgcga cggagcgttt ctaatgtact agatggcttc
      241 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      301 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      361 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      421 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
      481 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
      541 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
      601 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
      661 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
      721 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
      781 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
      841 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
      901 cggtgcttaa taacgctgat agtgctagtg tagatcgc
//