BBa_J100066
1
BBa_J100066
Synthetic Riboswitch
2012-06-11T11:00:00Z
2015-08-31T04:08:22Z
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed
This part is a synthetic riboswitch that can be used in E. coli. In the presence of theophylline, the expression of the gene of interest is induced, but in the absence of theophylline, very little transcription takes place. This part contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch.
Because the riboswitch must be right beside the gene of interest (the start codon is folded in the riboswitch), we added a BsaI site. This allows the riboswitch to be attached to the gene of interest using Golden Gate Assembly (as long as the first nucleotide after the start codon of the gene of interest is a C, we designed the part to use with superfolder GFP).
This riboswitch is modified from riboswitch E as described in the paper by Topp et al.
References: Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Doroth??e Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, and Justin P. Gallivan. Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species. Applied and Environmental Microbiology, 76:23, 7881-7884. December 2010.
false
false
_578_
0
10704
9
Not in stock
false
None
false
Rebecca Evans
annotation2176247
1
lac operator
range2176247
1
76
96
annotation2176245
1
BBa prefix
range2176245
1
1
22
annotation2176253
1
RBS
range2176253
1
168
174
annotation2176252
1
Start
range2176252
1
185
187
annotation2176251
1
Riboswitch
range2176251
1
125
187
annotation2176255
1
BBa suffix
range2176255
1
196
216
annotation2176254
1
GGA suffix ATGC sticky word
range2176254
1
185
195
annotation2176250
1
KpnI site
range2176250
1
119
124
annotation2176248
1
scar
range2176248
1
97
102
annotation2176249
1
constant sequence
range2176249
1
104
118
annotation2176246
1
T5 promoter
range2176246
1
23
75
BBa_J100066_sequence
1
gaattcgcggccgcttctagagaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactagagatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacaacaagatgctgagacctactagtagcggccgctgcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z