LOCUS       BBa_J100082             1276 bp    DNA     linear       18-May-2024
DEFINITION  J100072+E0240
ACCESSION   BBa_J100082
VERSION     BBa_J100082.1  
FEATURES             Location/Qualifiers
     misc_feature    329..343
                     /label=CpxR-phosphorylated
     misc_feature    349..363
                     /label=CpxR-phosphorylated
     misc_feature    374..392
                     /label=CpxR-phosphorylated
     promoter        1..392
                     /label=BBa_J100072
     RBS             401..413
                     /label=RBS-3\Weak
     RBS             407..410
                     /label=RBS
     misc_feature    401..413
                     /label=BBa_B0032
     RBS             401..413
                     /label=BBa_B0032
     CDS             420..1139
                     /label=GFP protein
     CDS             420..1139
                     /label=BBa_E0040
     stem_loop       1159..1202
                     /label=stem_loop
     misc_feature    1148..1227
                     /label=BBa_B0010
     terminator      1148..1227
                     /label=BBa_B0010
     stem_loop       1243..1262
                     /label=T7 TE
     misc_feature    1269..1269
                     /label=stop
     polyA_site      1263..1276
                     /label=polya
     misc_feature    1236..1276
                     /label=BBa_B0012
     terminator      1236..1276
                     /label=BBa_B0012
     misc_feature    401..1276
                     /label=BBa_E0240
ORIGIN
        1 ctcaaggccg agaacgcgat caagttcact gccgcgcgcc gtcaacataa tgacaggcgt
       61 ctggtgtgtc tggcgaagtg cttttaatgt gtcgatacca tttttcttcg gcatcattac
      121 gtcaagcaaa agtaaatcaa tgctgtcgtc cagaagatca agcgcctgtt ccccatcgtg
      181 ggcaacaatc acgttgaagc cttccatctc gagcagctcc tttaataggg aagtcagctc
      241 tcggtcatca tcaactaaca ggattttatt cattgtttaa atacctccga ggcagaaatt
      301 acgtcatcag acgtcgctaa tccatgactt tacgttgttt tacaccccct gacgcatgtt
      361 tgcagcctga atcgtaaact ctctatcgtt gatactagag tcacacagga aagtactaga
      421 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      481 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      541 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      601 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      661 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      721 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      781 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      841 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      901 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      961 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1021 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1081 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1141 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1201 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1261 cctttctgcg tttata
//