##gff-version 3
##sequence-region BBa_J100082 1 1276
BBa_J100082	.	engineered_region	401	1276	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_J100082	.	terminator	1148	1227	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_J100082	.	stem_loop	1159	1202	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100082	.	engineered_region	1148	1227	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100082	.	terminator	1236	1276	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_J100082	.	stem_loop	1243	1262	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100082	.	polyA_site	1263	1276	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100082	.	stop_codon	1269	1269	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100082	.	engineered_region	1236	1276	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100082	.	CDS	420	1139	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_J100082	.	CDS	420	1139	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100082	.	ribosome_entry_site	401	413	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_J100082	.	engineered_region	401	413	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J100082	.	ribosome_entry_site	407	410	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J100082	.	ribosome_entry_site	401	413	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J100082	.	promoter	1	392	.	+	0	ID=BBa_J100072;Name=BBa_J100072
BBa_J100082	.	non_covalent_binding_site	349	363	.	+	0	ID=annotation2177468;Name=CpxR-phosphorylated;Parent=BBa_J100072
BBa_J100082	.	non_covalent_binding_site	374	392	.	+	0	ID=annotation2177469;Name=CpxR-phosphorylated;Parent=BBa_J100072
BBa_J100082	.	non_covalent_binding_site	329	343	.	+	0	ID=annotation2177465;Name=CpxR-phosphorylated;Parent=BBa_J100072
>BBa_J100082
ctcaaggccgagaacgcgatcaagttcactgccgcgcgccgtcaacataatgacaggcgtctggtgtgtctggcgaagt
gcttttaatgtgtcgataccatttttcttcggcatcattacgtcaagcaaaagtaaatcaatgctgtcgtccagaagat
caagcgcctgttccccatcgtgggcaacaatcacgttgaagccttccatctcgagcagctcctttaatagggaagtcag
ctctcggtcatcatcaactaacaggattttattcattgtttaaatacctccgaggcagaaattacgtcatcagacgtcg
ctaatccatgactttacgttgttttacaccccctgacgcatgtttgcagcctgaatcgtaaactctctatcgttgatac
tagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata