##gff-version 3
##sequence-region BBa_J100086 1 1035
BBa_J100086	.	engineered_region	160	1035	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_J100086	.	terminator	995	1035	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_J100086	.	engineered_region	995	1035	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J100086	.	stop_codon	1028	1028	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J100086	.	polyA_site	1022	1035	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J100086	.	stem_loop	1002	1021	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J100086	.	terminator	907	986	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_J100086	.	stem_loop	918	961	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J100086	.	engineered_region	907	986	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J100086	.	CDS	179	898	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_J100086	.	CDS	179	898	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J100086	.	ribosome_entry_site	160	172	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_J100086	.	ribosome_entry_site	166	169	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J100086	.	ribosome_entry_site	160	172	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J100086	.	engineered_region	160	172	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J100086	.	promoter	1	151	.	+	0	ID=BBa_J100076;Name=BBa_J100076
BBa_J100086	.	non_covalent_binding_site	35	84	.	+	0	ID=annotation2177487;Name=arcA binding site;Parent=BBa_J100076
BBa_J100086	.	non_covalent_binding_site	91	105	.	+	0	ID=annotation2177488;Name=Fnr binding site;Parent=BBa_J100076
BBa_J100086	.	sequence_feature	116	116	.	+	0	ID=annotation2177489;Name=-35;Parent=BBa_J100076
BBa_J100086	.	sequence_feature	141	141	.	+	0	ID=annotation2177486;Name=-10;Parent=BBa_J100076
BBa_J100086	.	sequence_feature	151	151	.	+	0	ID=annotation2177485;Name=+1;Parent=BBa_J100076
>BBa_J100086
aactaatttcagccttataactcacacattttaaacataaatgtcagtaaagttaccttattgaaacatgattaacata
atttgtaggaattgatatttatcaatgtataagtcttggaaatgggcatcaaaaagagataaattgttctcgtactaga
gtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatgg
tgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcga
gatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatt
tttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtta
aaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtataca
tcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaact
agcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaa
tctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacac
atggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata