LOCUS       BBa_J100088             2965 bp    DNA     linear       18-Jun-2024
DEFINITION  J100071+J10063
ACCESSION   BBa_J100088
VERSION     BBa_J100088.1  
FEATURES             Location/Qualifiers
     misc_feature    11..25
                     /label=H-NS binding site
     misc_feature    68..82
                     /label=H-NS binding site
     misc_feature    149..163
                     /label=H-NS binding site
     misc_feature    153..167
                     /label=H-NS binding site
     misc_feature    199..213
                     /label=H-NS binding site
     misc_feature    296..310
                     /label=H-NS binding site
     misc_feature    188..225
                     /label=Cad1 binding site 
     misc_feature    249..279
                     /label=Cad2 binding site
     misc_feature    320..326
                     /label=-10
     misc_feature    298..306
                     /label=-35
     promoter        1..334
                     /label=BBa_J100071
     RBS             343..359
                     /label=endogenous RBS
     misc_feature    1287..1292
                     /label=double stop
     misc_feature    360..362
                     /label=LRE ORF
     CDS             360..1292
                     /label=Luciferin Regenerating Enzyme
     misc_feature    1293..1298
                     /label=BamH 1 site
     RBS             1299..1315
                     /label=endogenous RBS
     misc_feature    1316..1318
                     /label=green luciferase ATG
     CDS             1316..2965
                     /label=green luciferase
     misc_feature    2960..2965
                     /label=double stop
     misc_feature    2170..2170
                     /label=T --> C kill Spe I site
     misc_feature    343..2965
                     /label=BBa_J10063
ORIGIN
        1 ccttatgttg taccttatct cgacaaattt cttgcttcag aataagtaac tccgggttga
       61 tttatgctcg gaaatatttg ttgttgagtt tttgtatgtt cctgttggta taatatgttg
      121 cggcaattta tttgccgcat aatttttatt acataaattt aaccagagaa tgtcacgcaa
      181 tccattgtaa acattaaatg tttatctttt catgatatca acttgcgatc ctgatgtgtt
      241 aataaaaaac ctcaagttct cacttacaga aacttttgtg ttatttcacc taatctttag
      301 gattaatcct tttttcgtga gtaatcttat cgcctactag agttaaggag gtaaaaaaaa
      361 tggccccgac cgtggaacaa attgtggagc tgggtacgta tctgctggcc gaaagcccgc
      421 actgggacga tgaaacgcaa agcctgtatt tcgtcgatat cgttggtcgc agcgtgaaca
      481 aatacgttcc gacgaccaag acgcataccc aactgaaatt cgacaagaat ccgagcttca
      541 tcatcccggt gaagggttgt tccgaccgtt ttatcgtcag cctggaacgc gagatcaacc
      601 tgctgacctg ggacggcgca tcctctgcac catccaagat tgagaagatc gcggttttcg
      661 ataatacgcc agagaagagc gaaaaccgtc tgaacgatgg taaggcagat ccgctgggca
      721 acctgtgggc gggtaccatg aatatgggtt ctgaccatac caccggcacg ccggtgcgcg
      781 gtaccctgag cagcctgagc aacaagcaag tcaaagagca cgtcagcgag gtctgcatca
      841 gcaatggcct ggcatggagc aaggacctga agaaattcta ttacattgat agcgctgttc
      901 gtcaagttga ccagttcgat ttcgatgcga aaaacctgag cttgagcaat cgtcaaccgc
      961 tgttcacctt tgacaaacac ggcatcatgg gtagcccgga tggtcagacc atcgatgcgg
     1021 agggtaacct gtgggttgcc acctgtcagg gcgataaagt cctgaagatt gatacttcta
     1081 ccccggaaac cctgctgggt attgtggaga ttccggaaca tcaggtgacg agcgtttgca
     1141 tcggcggtgc agaactgaac gttttgtatg tgacgaccgc gtccattaaa ctgcctggtg
     1201 cggatgaaac caagccgatg aaaggcgcca tctataaagt caccggtctg ggtgtcaaag
     1261 gtctgccggg tgaccgtgtt aagctgtaat aaggatcctt aaggaggtaa aaaaaatgga
     1321 gaacatggag aacgacgaga atattgtggt tggtccgaaa ccgttctacc cgatcgaaga
     1381 gggcagcgcg ggtacccagc tgcgtaaata catggaacgc tacgcgaaac tgggtgcgat
     1441 cgcgttcacg aatgcggtta ccggcgtgga ctattcctat gcggagtatt tggaaaagtc
     1501 gtgttgtctg ggcaaggcgt tacagaatta tggcctggtg gtcgatggcc gtatcgcgct
     1561 gtgcagcgag aactgtgaag agtttttcat tccggtgatc gcgggtttgt ttattggcgt
     1621 gggcgttgca ccgaccaatg aaatctacac gctgcgcgaa ctggtgcact ccctgggtat
     1681 ctctaagccg acgatcgttt tcagctccaa gaaaggtttg gataaagtta ttacggtgca
     1741 aaagacggtc accacgatca aaacgattgt gatcctggac agcaaagttg attaccgcgg
     1801 ttatcaatgc ctggatacgt tcatcaagcg taacaccccg ccaggttttc aagcatctag
     1861 cttcaaaacg gtggaggttg accgtaaaga acaagtcgca ctgattatga atagctcggg
     1921 tagcacgggc ctgccaaagg gtgtgcagct gacccatgaa aacaccgtta cccgttttag
     1981 ccacgcgcgt gatccgattt acggcaatca agtgtcgccg ggtacggcta ttctgactgt
     2041 tgtccctttt caccatggtt ttggcatgtt taccactctg ggttacctga tttgcggttt
     2101 tcgcgtcgtt atgctgacca agtttgatga agaaaccttc ctgaaaaccc tgcaagacta
     2161 caagtgcacc agtgtgattc tggtcccgac gctgttcgcg atcctgaaca aaagcgagct
     2221 gctgaataag tacgacctga gcaatttggt ggagattgcg agcggcggtg ctccgttgag
     2281 caaagaagta ggcgaggcag tggcacgtcg ctttaacctg cctggcgtcc gtcaaggtta
     2341 cggtttgact gaaaccacga gcgcgattat catcaccccg gaaggtgacg ataaacctgg
     2401 cgctagcggt aaggtcgtgc cgttgttcaa ggctaaagtt attgatctgg acaccaagaa
     2461 gagcctgggc ccgaatcgcc gtggtgaagt ttgcgttaag ggcccgatgc tgatgaaagg
     2521 ttatgtaaac aatccggaag caacgaaaga gctgatcgac gaagagggct ggctgcacac
     2581 tggcgatatc ggctactacg atgaagagaa acactttttc attgttgatc gcctgaaaag
     2641 cctgatcaag tacaagggtt accaagtccc gccagcggag ctggaaagcg tactgttgca
     2701 gcacccgtcg attttcgacg ccggtgtggc tggtgtgcct gatccggtgg ctggcgaact
     2761 gccgggtgct gtcgtggtgc tggagagcgg taagaatatg accgaaaaag aagtcatgga
     2821 ctatgtggcg agccaagtgt ccaatgcaaa acgtctgcgt ggcggtgttc gctttgtgga
     2881 cgaagtgccg aaaggtctga ccggcaaaat tgacggtcgt gcaattcgtg agattctgaa
     2941 gaaaccggtc gcgaaaatgt aataa
//