BBa_J10063 1 BBa_J10063 Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised) 2012-01-10T12:00:00Z 2015-08-31T04:08:24Z from registry, synthesized de novo. The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA. This part now starts with the RBS in part K325229. K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence. false false _578_120_ 0 201 61 Not in stock false Wanted to allow users to use their favorite promoter. false Malcolm Campbell annotation2169570 1 endogenous RBS range2169570 1 957 973 annotation2169569 1 BamH 1 site range2169569 1 951 956 annotation2169567 1 LRE ORF range2169567 1 18 20 annotation2169574 1 T --> C kill Spe I site range2169574 1 1828 1828 annotation2169571 1 green luciferase ATG range2169571 1 974 976 annotation2169566 1 double stop range2169566 1 945 950 annotation2169565 1 endogenous RBS range2169565 1 1 17 annotation2169568 1 Luciferin Regenerating Enzyme range2169568 1 18 950 annotation2169573 1 double stop range2169573 1 2618 2623 annotation2169572 1 green luciferase range2169572 1 974 2623 BBa_J100071 1 BBa_J100071 cadA promoter 2012-07-04T11:00:00Z 2015-08-31T04:08:22Z Taken from ''E. coli'' genome through PCR. The promoter for the cadBA operon in ''E. coli''. It is induced by low pH ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=EG10131 cadA gene]). CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00436 CadC function in cadBA operon]). false true _578_ 0 13950 9 Not in stock false We cloned the promoter region 334 bp upstream of the transcription start site, in which Cad1 and Cad2 are included. false Ben Clarkson annotation2177463 1 Cad1 binding site range2177463 1 188 225 annotation2177458 1 H-NS binding site range2177458 1 68 82 annotation2177467 1 -35 range2177467 1 298 306 annotation2177461 1 H-NS binding site range2177461 1 199 213 annotation2177466 1 -10 range2177466 1 320 326 annotation2177459 1 H-NS binding site range2177459 1 149 163 annotation2177457 1 H-NS binding site range2177457 1 11 25 annotation2177462 1 H-NS binding site range2177462 1 296 310 annotation2177460 1 H-NS binding site range2177460 1 153 167 annotation2177464 1 Cad2 binding site range2177464 1 249 279 BBa_J100088 1 BBa_J100088 J100071+J10063 2012-07-17T11:00:00Z 2015-08-31T04:08:22Z Promoter from E. coli geonome, Green Firefly luciferase from L. Cruciata [http://partsregistry.org/Part:BBa_J100071 J100071]+[http://partsregistry.org/Part:BBa_J10063 J10063] Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised) under control of cadA promoter. false false _578_ 0 13950 9 Not in stock false Promoter includes Cad1 and Cad2 binding sites. Coding sequence contains Luciferin Regenerating Enzyme and Luciferase coding sequences. false Ben Clarkson component2177753 1 BBa_J10063 component2177742 1 BBa_J100071 annotation2177753 1 BBa_J10063 range2177753 1 343 2965 annotation2177742 1 BBa_J100071 range2177742 1 1 334 BBa_J10063_sequence 1 ttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctattctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcaccagtgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa BBa_J100071_sequence 1 ccttatgttgtaccttatctcgacaaatttcttgcttcagaataagtaactccgggttgatttatgctcggaaatatttgttgttgagtttttgtatgttcctgttggtataatatgttgcggcaatttatttgccgcataatttttattacataaatttaaccagagaatgtcacgcaatccattgtaaacattaaatgtttatcttttcatgatatcaacttgcgatcctgatgtgttaataaaaaacctcaagttctcacttacagaaacttttgtgttatttcacctaatctttaggattaatccttttttcgtgagtaatcttatcgcc BBa_J100088_sequence 1 ccttatgttgtaccttatctcgacaaatttcttgcttcagaataagtaactccgggttgatttatgctcggaaatatttgttgttgagtttttgtatgttcctgttggtataatatgttgcggcaatttatttgccgcataatttttattacataaatttaaccagagaatgtcacgcaatccattgtaaacattaaatgtttatcttttcatgatatcaacttgcgatcctgatgtgttaataaaaaacctcaagttctcacttacagaaacttttgtgttatttcacctaatctttaggattaatccttttttcgtgagtaatcttatcgcctactagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctattctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcaccagtgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z