##gff-version 3
##sequence-region BBa_J100088 1 2965
BBa_J100088	.	promoter	1	334	.	+	0	ID=BBa_J100071;Name=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	153	167	.	+	0	ID=annotation2177460;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	68	82	.	+	0	ID=annotation2177458;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	296	310	.	+	0	ID=annotation2177462;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	298	306	.	+	0	ID=annotation2177467;Name=-35;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	11	25	.	+	0	ID=annotation2177457;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	188	225	.	+	0	ID=annotation2177463;Name=Cad1 binding site ;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	249	279	.	+	0	ID=annotation2177464;Name=Cad2 binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	320	326	.	+	0	ID=annotation2177466;Name=-10;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	199	213	.	+	0	ID=annotation2177461;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	non_covalent_binding_site	149	163	.	+	0	ID=annotation2177459;Name=H-NS binding site;Parent=BBa_J100071
BBa_J100088	.	engineered_region	343	2965	.	+	0	ID=BBa_J10063;Name=BBa_J10063
BBa_J100088	.	ribosome_entry_site	343	359	.	+	0	ID=annotation2169565;Name=endogenous RBS;Parent=BBa_J10063
BBa_J100088	.	start_codon	1316	1318	.	+	0	ID=annotation2169571;Name=green luciferase ATG;Parent=BBa_J10063
BBa_J100088	.	stop_codon	2960	2965	.	+	0	ID=annotation2169573;Name=double stop;Parent=BBa_J10063
BBa_J100088	.	start_codon	360	362	.	+	0	ID=annotation2169567;Name=LRE ORF;Parent=BBa_J10063
BBa_J100088	.	point_mutation	2170	2170	.	+	0	ID=annotation2169574;Name=T --> C kill Spe I site;Parent=BBa_J10063
BBa_J100088	.	stop_codon	1287	1292	.	+	0	ID=annotation2169566;Name=double stop;Parent=BBa_J10063
BBa_J100088	.	sequence_feature	1293	1298	.	+	0	ID=annotation2169569;Name=BamH 1 site;Parent=BBa_J10063
BBa_J100088	.	CDS	360	1292	.	+	0	ID=annotation2169568;Name=Luciferin Regenerating Enzyme;Parent=BBa_J10063
BBa_J100088	.	CDS	1316	2965	.	+	0	ID=annotation2169572;Name=green luciferase;Parent=BBa_J10063
BBa_J100088	.	ribosome_entry_site	1299	1315	.	+	0	ID=annotation2169570;Name=endogenous RBS;Parent=BBa_J10063
>BBa_J100088
ccttatgttgtaccttatctcgacaaatttcttgcttcagaataagtaactccgggttgatttatgctcggaaatattt
gttgttgagtttttgtatgttcctgttggtataatatgttgcggcaatttatttgccgcataatttttattacataaat
ttaaccagagaatgtcacgcaatccattgtaaacattaaatgtttatcttttcatgatatcaacttgcgatcctgatgt
gttaataaaaaacctcaagttctcacttacagaaacttttgtgttatttcacctaatctttaggattaatccttttttc
gtgagtaatcttatcgcctactagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggt
acgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcg
tgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaa
gggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcacca
tccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatc
cgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgag
cagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctg
aagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagca
atcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaa
cctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtg
gagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtcca
ttaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtct
gccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaata
ttgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgcta
cgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcg
tgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaag
agtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcga
actggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtg
caaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggata
cgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagt
cgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgt
tttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctattctgactgttgtcccttttcacc
atggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaaga
aaccttcctgaaaaccctgcaagactacaagtgcaccagtgtgattctggtcccgacgctgttcgcgatcctgaacaaa
agcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtag
gcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattat
catcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctg
gacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaa
acaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaaga
gaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaa
agcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgg
gtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaa
tgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgca
attcgtgagattctgaagaaaccggtcgcgaaaatgtaataa