LOCUS       BBa_J100089             3023 bp    DNA     linear       26-Nov-2024
DEFINITION  J100072+J10063 (LcpxP+LRE, Luciferase)
ACCESSION   BBa_J100089
VERSION     BBa_J100089.1  
FEATURES             Location/Qualifiers
     misc_feature    329..343
                     /label=CpxR-phosphorylated
     misc_feature    349..363
                     /label=CpxR-phosphorylated
     misc_feature    374..392
                     /label=CpxR-phosphorylated
     promoter        1..392
                     /label=BBa_J100072
     RBS             401..417
                     /label=endogenous RBS
     misc_feature    1345..1350
                     /label=double stop
     misc_feature    418..420
                     /label=LRE ORF
     CDS             418..1350
                     /label=Luciferin Regenerating Enzyme
     misc_feature    1351..1356
                     /label=BamH 1 site
     RBS             1357..1373
                     /label=endogenous RBS
     misc_feature    1374..1376
                     /label=green luciferase ATG
     CDS             1374..3023
                     /label=green luciferase
     misc_feature    3018..3023
                     /label=double stop
     misc_feature    2228..2228
                     /label=T --> C kill Spe I site
     misc_feature    401..3023
                     /label=BBa_J10063
ORIGIN
        1 ctcaaggccg agaacgcgat caagttcact gccgcgcgcc gtcaacataa tgacaggcgt
       61 ctggtgtgtc tggcgaagtg cttttaatgt gtcgatacca tttttcttcg gcatcattac
      121 gtcaagcaaa agtaaatcaa tgctgtcgtc cagaagatca agcgcctgtt ccccatcgtg
      181 ggcaacaatc acgttgaagc cttccatctc gagcagctcc tttaataggg aagtcagctc
      241 tcggtcatca tcaactaaca ggattttatt cattgtttaa atacctccga ggcagaaatt
      301 acgtcatcag acgtcgctaa tccatgactt tacgttgttt tacaccccct gacgcatgtt
      361 tgcagcctga atcgtaaact ctctatcgtt gatactagag ttaaggaggt aaaaaaaatg
      421 gccccgaccg tggaacaaat tgtggagctg ggtacgtatc tgctggccga aagcccgcac
      481 tgggacgatg aaacgcaaag cctgtatttc gtcgatatcg ttggtcgcag cgtgaacaaa
      541 tacgttccga cgaccaagac gcatacccaa ctgaaattcg acaagaatcc gagcttcatc
      601 atcccggtga agggttgttc cgaccgtttt atcgtcagcc tggaacgcga gatcaacctg
      661 ctgacctggg acggcgcatc ctctgcacca tccaagattg agaagatcgc ggttttcgat
      721 aatacgccag agaagagcga aaaccgtctg aacgatggta aggcagatcc gctgggcaac
      781 ctgtgggcgg gtaccatgaa tatgggttct gaccatacca ccggcacgcc ggtgcgcggt
      841 accctgagca gcctgagcaa caagcaagtc aaagagcacg tcagcgaggt ctgcatcagc
      901 aatggcctgg catggagcaa ggacctgaag aaattctatt acattgatag cgctgttcgt
      961 caagttgacc agttcgattt cgatgcgaaa aacctgagct tgagcaatcg tcaaccgctg
     1021 ttcacctttg acaaacacgg catcatgggt agcccggatg gtcagaccat cgatgcggag
     1081 ggtaacctgt gggttgccac ctgtcagggc gataaagtcc tgaagattga tacttctacc
     1141 ccggaaaccc tgctgggtat tgtggagatt ccggaacatc aggtgacgag cgtttgcatc
     1201 ggcggtgcag aactgaacgt tttgtatgtg acgaccgcgt ccattaaact gcctggtgcg
     1261 gatgaaacca agccgatgaa aggcgccatc tataaagtca ccggtctggg tgtcaaaggt
     1321 ctgccgggtg accgtgttaa gctgtaataa ggatccttaa ggaggtaaaa aaaatggaga
     1381 acatggagaa cgacgagaat attgtggttg gtccgaaacc gttctacccg atcgaagagg
     1441 gcagcgcggg tacccagctg cgtaaataca tggaacgcta cgcgaaactg ggtgcgatcg
     1501 cgttcacgaa tgcggttacc ggcgtggact attcctatgc ggagtatttg gaaaagtcgt
     1561 gttgtctggg caaggcgtta cagaattatg gcctggtggt cgatggccgt atcgcgctgt
     1621 gcagcgagaa ctgtgaagag tttttcattc cggtgatcgc gggtttgttt attggcgtgg
     1681 gcgttgcacc gaccaatgaa atctacacgc tgcgcgaact ggtgcactcc ctgggtatct
     1741 ctaagccgac gatcgttttc agctccaaga aaggtttgga taaagttatt acggtgcaaa
     1801 agacggtcac cacgatcaaa acgattgtga tcctggacag caaagttgat taccgcggtt
     1861 atcaatgcct ggatacgttc atcaagcgta acaccccgcc aggttttcaa gcatctagct
     1921 tcaaaacggt ggaggttgac cgtaaagaac aagtcgcact gattatgaat agctcgggta
     1981 gcacgggcct gccaaagggt gtgcagctga cccatgaaaa caccgttacc cgttttagcc
     2041 acgcgcgtga tccgatttac ggcaatcaag tgtcgccggg tacggctatt ctgactgttg
     2101 tcccttttca ccatggtttt ggcatgttta ccactctggg ttacctgatt tgcggttttc
     2161 gcgtcgttat gctgaccaag tttgatgaag aaaccttcct gaaaaccctg caagactaca
     2221 agtgcaccag tgtgattctg gtcccgacgc tgttcgcgat cctgaacaaa agcgagctgc
     2281 tgaataagta cgacctgagc aatttggtgg agattgcgag cggcggtgct ccgttgagca
     2341 aagaagtagg cgaggcagtg gcacgtcgct ttaacctgcc tggcgtccgt caaggttacg
     2401 gtttgactga aaccacgagc gcgattatca tcaccccgga aggtgacgat aaacctggcg
     2461 ctagcggtaa ggtcgtgccg ttgttcaagg ctaaagttat tgatctggac accaagaaga
     2521 gcctgggccc gaatcgccgt ggtgaagttt gcgttaaggg cccgatgctg atgaaaggtt
     2581 atgtaaacaa tccggaagca acgaaagagc tgatcgacga agagggctgg ctgcacactg
     2641 gcgatatcgg ctactacgat gaagagaaac actttttcat tgttgatcgc ctgaaaagcc
     2701 tgatcaagta caagggttac caagtcccgc cagcggagct ggaaagcgta ctgttgcagc
     2761 acccgtcgat tttcgacgcc ggtgtggctg gtgtgcctga tccggtggct ggcgaactgc
     2821 cgggtgctgt cgtggtgctg gagagcggta agaatatgac cgaaaaagaa gtcatggact
     2881 atgtggcgag ccaagtgtcc aatgcaaaac gtctgcgtgg cggtgttcgc tttgtggacg
     2941 aagtgccgaa aggtctgacc ggcaaaattg acggtcgtgc aattcgtgag attctgaaga
     3001 aaccggtcgc gaaaatgtaa taa
//