##gff-version 3
##sequence-region BBa_J100089 1 3023
BBa_J100089	.	engineered_region	401	3023	.	+	0	ID=BBa_J10063;Name=BBa_J10063
BBa_J100089	.	ribosome_entry_site	1357	1373	.	+	0	ID=annotation2169570;Name=endogenous RBS;Parent=BBa_J10063
BBa_J100089	.	sequence_feature	1351	1356	.	+	0	ID=annotation2169569;Name=BamH 1 site;Parent=BBa_J10063
BBa_J100089	.	start_codon	418	420	.	+	0	ID=annotation2169567;Name=LRE ORF;Parent=BBa_J10063
BBa_J100089	.	start_codon	1374	1376	.	+	0	ID=annotation2169571;Name=green luciferase ATG;Parent=BBa_J10063
BBa_J100089	.	point_mutation	2228	2228	.	+	0	ID=annotation2169574;Name=T --> C kill Spe I site;Parent=BBa_J10063
BBa_J100089	.	ribosome_entry_site	401	417	.	+	0	ID=annotation2169565;Name=endogenous RBS;Parent=BBa_J10063
BBa_J100089	.	CDS	1374	3023	.	+	0	ID=annotation2169572;Name=green luciferase;Parent=BBa_J10063
BBa_J100089	.	stop_codon	3018	3023	.	+	0	ID=annotation2169573;Name=double stop;Parent=BBa_J10063
BBa_J100089	.	stop_codon	1345	1350	.	+	0	ID=annotation2169566;Name=double stop;Parent=BBa_J10063
BBa_J100089	.	CDS	418	1350	.	+	0	ID=annotation2169568;Name=Luciferin Regenerating Enzyme;Parent=BBa_J10063
BBa_J100089	.	promoter	1	392	.	+	0	ID=BBa_J100072;Name=BBa_J100072
BBa_J100089	.	non_covalent_binding_site	374	392	.	+	0	ID=annotation2177469;Name=CpxR-phosphorylated;Parent=BBa_J100072
BBa_J100089	.	non_covalent_binding_site	349	363	.	+	0	ID=annotation2177468;Name=CpxR-phosphorylated;Parent=BBa_J100072
BBa_J100089	.	non_covalent_binding_site	329	343	.	+	0	ID=annotation2177465;Name=CpxR-phosphorylated;Parent=BBa_J100072
>BBa_J100089
ctcaaggccgagaacgcgatcaagttcactgccgcgcgccgtcaacataatgacaggcgtctggtgtgtctggcgaagt
gcttttaatgtgtcgataccatttttcttcggcatcattacgtcaagcaaaagtaaatcaatgctgtcgtccagaagat
caagcgcctgttccccatcgtgggcaacaatcacgttgaagccttccatctcgagcagctcctttaatagggaagtcag
ctctcggtcatcatcaactaacaggattttattcattgtttaaatacctccgaggcagaaattacgtcatcagacgtcg
ctaatccatgactttacgttgttttacaccccctgacgcatgtttgcagcctgaatcgtaaactctctatcgttgatac
tagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtggagctgggtacgtatctgctggccgaaagc
ccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggtcgcagcgtgaacaaatacgttccgacga
ccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcccggtgaagggttgttccgaccgttttat
cgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctctgcaccatccaagattgagaagatcgcg
gttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaaggcagatccgctgggcaacctgtgggcgg
gtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggtaccctgagcagcctgagcaacaagcaagt
caaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaaggacctgaagaaattctattacattgat
agcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagcttgagcaatcgtcaaccgctgttcacct
ttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcggagggtaacctgtgggttgccacctgtca
gggcgataaagtcctgaagattgatacttctaccccggaaaccctgctgggtattgtggagattccggaacatcaggtg
acgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgaccgcgtccattaaactgcctggtgcggatg
aaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtcaaaggtctgccgggtgaccgtgttaagct
gtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgt
tctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgc
gttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgtta
cagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcg
cgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtat
ctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatc
aaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccc
cgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctc
gggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccg
atttacggcaatcaagtgtcgccgggtacggctattctgactgttgtcccttttcaccatggttttggcatgtttacca
ctctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgca
agactacaagtgcaccagtgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtac
gacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgct
ttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacga
taaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggc
ccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaag
agctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttga
tcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccg
tcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggaga
gcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcgg
tgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaa
ccggtcgcgaaaatgtaataa