##gff-version 3
##sequence-region BBa_J100091 1 926
BBa_J100091	.	ribosome_entry_site	157	165	.	+	0	ID=annotation2180194;Name=leader RBS
BBa_J100091	.	sequence_feature	23	26	.	+	0	ID=annotation2180188;Name=BsaI sticky end left
BBa_J100091	.	stop_codon	223	225	.	+	0	ID=annotation2180196;Name=Stop for BD18 leader
BBa_J100091	.	sequence_feature	140	227	.	+	0	ID=annotation2180193;Name=BD18 bicistron
BBa_J100091	.	stem_loop	34	128	.	+	0	ID=annotation2180190;Name=TT B0014
BBa_J100091	.	start_codon	225	227	.	+	0	ID=annotation2180197;Name=Start for RFP
BBa_J100091	.	ribosome_entry_site	210	218	.	+	0	ID=annotation2180195;Name=RBS for RFP
BBa_J100091	.	primer_binding_site	232	251	.	+	0	ID=annotation2330898;Name=PCR Rev Primer
BBa_J100091	.	stop_codon	900	905	.	+	0	ID=annotation2180199;Name= Double Stop for E1010
BBa_J100091	.	sequence_feature	28	33	.	+	0	ID=annotation2180189;Name=BsaI cuts left
BBa_J100091	.	sequence_feature	129	134	.	+	0	ID=annotation2180191;Name=BsaI cuts right
BBa_J100091	.	primer_binding_site	1	20	.	+	0	ID=annotation2330897;Name=PCR For Primer
BBa_J100091	.	engineered_region	906	926	.	+	0	ID=annotation2180200;Name=BBa suffix
BBa_J100091	.	sequence_feature	136	139	.	+	0	ID=annotation2180192;Name=BsaI sticky end right
BBa_J100091	.	engineered_region	1	22	.	+	0	ID=annotation2180187;Name=BBa Prefix
BBa_J100091	.	CDS	225	905	.	+	0	ID=annotation2180198;Name=RFP E1010
>BBa_J100091
gaattcgcggccgcttctagagcgactgagacctcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagagagaatataaaaagccagattattaatccggcttttttattatttggtctcagcgggggcccaagttcacttaaa
aaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccg
aagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaagg
tgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgct
tgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctga
aactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccagga
ctcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatg
cagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaa
tgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagct
gccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaa
cgtgctgaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcag