LOCUS BBa_J100100 3204 bp DNA linear 22-Dec-2024 DEFINITION E. coli optimized CDM ACCESSION BBa_J100100 VERSION BBa_J100100.1 FEATURES Location/Qualifiers misc_feature 1..22 /label=Bba_prefix misc_feature 3184..3204 /label=Bba_suffix misc_feature 31..33 /label=start misc_feature 3178..3183 /label=Stop misc_feature 23..28 /label=BsaI cuts right misc_feature 30..33 /label=BsaI sticky end ORIGIN 1 gaattcgcgg ccgcttctag agggtctcga atgaccatca aagaaatgcc gcagccgaaa 61 acctttggtg aactgaaaaa tctgccgctg ctgaataccg ataaaccggt tcatgcactg 121 atgaaaattg cagatgaact gggcgagatc tttaaattcg aagcaccggg tagcgttacc 181 cgttatctga gcagccagcg tctgattaaa gaagcatgcg acgaaagccg ctttgataaa 241 aatctgattc agtggctgaa attcatccgc gattttctgg gtgatggtct gagcaccagc 301 tggacccatg aaaaaaactg gaaaaaagcc cataatattc tgctgccgag ctttagccag 361 caggcaatga aaggttatca tgcaatgatg gttgatattg ccgttcagct ggttcagaaa 421 tgggaacgtc tgaatgcaga tgaacatatt gaagttccgg aagatatgac ccgtctgacc 481 ctggatacca ttggtctgtg tggttttaac tatcgcttca acagctttta tcgtgatcag 541 ccgcatccgt ttgttaccag catggttcgt gcactggatg aagcagtgaa taaatggcag 601 cgtgcaaatc cggatgatcc ggcatatgat gaaaataaac gtcagttcca agaggatatt 661 aaagtgatga atgacctggt ggacaaaatt atcgcagatc gtaaagcaag cggtgaacag 721 agtgatgatc tgctgaccca tatgctgaat ggtaaagatc cggaaaccgg tgaaccgctg 781 gatgatgaaa acattcgtta tcagattatt accttcctga ttgccggtca tgaaaccacc 841 agtggtctgc tgagctttgc actgtatttt ctggttaaaa atccgcacgt tctgcaaaaa 901 gcagccgaag aagcagcacg cgttctggtt gatccggttc cgagctataa acaggttaaa 961 cagctgaaat atgtgggcat ggttctgaat gaagcactgc gtctgtggcc gacctttccg 1021 tggtttagcc tgtatgcaaa agaagatacc gttctgggag gtgaatatcc gctggaaaaa 1081 ggtgatgaac tgatggttct gattccgcag ctgcatcgtg ataaaaccat ttggggtgat 1141 gatgtggaag aatttcgtcc ggaacgtttt gaaaatccga gcgcaattcc gcagcatgcc 1201 tttaaaccgt ttggtaatgg tcagcgtgca tgtattggtc agcagtttgc actgcatgaa 1261 gccaccctgg ttctgggtat gatgctgaaa cattttgatt ttgaggacca caccaactat 1321 gagctggata ttaaaggcac cctgaccctg aaaccggaag gttttgttgt taaagccaaa 1381 agcaaaaaaa tcccgctggg tggtattccg agcccgagca ccgaacagag cgcaaaaaaa 1441 gttcgtaaaa aagccgaaaa cgcccataat acaccgctgc tggttctgta tggtagcaat 1501 atgggtacag ccgaaggcac cgcacgtgat ctggcagata ttgcaatgag caaaggtttt 1561 gcaccgcagg ttgcaaccct ggatagccat gcaggtaatc tgcctcgtga aggtgcagtt 1621 ctgattgtta ccgcaagcta taatggtcat ccgcctgata atgcaaaaca gtttgttgat 1681 tggctggatc aggcaagcgc agatgaagtt aaaggtgttc gttatagcgt ttttggttgc 1741 ggtgataaaa actgggcaac cacctatcag aaagttccgg catttattga tgaaaccctg 1801 gcagcaaaag gtgcagaaaa tattgcagat cgtggtgaaa ccgatgccag tgatgatttt 1861 gaaggcacct atgaagaatg gcgtgaacat atgtggtcag atgttgcagc ctattttaac 1921 ctggatatcg aaaacagcga ggacaataaa agcaccctga gcctgcaatt tgtggatagc 1981 gcagcagata tgccgctggc aaaaatgcat ggtgcattta gcaccaatgt tgttgcaagc 2041 aaagaactgc aacagcctgg tagcgcacgt agcacccgtc atctggaaat tgaactgccg 2101 aaagaagcaa gctatcaaga gggtgatcat ctgggtgtta ttccgcgtaa ttatgaaggt 2161 attgttaatc gtgttaccgc acgttttggt ctggatgcaa gccagcagat tcgtctggaa 2221 gcagaagaag aaaaactggc acatctgccg ctggccaaaa ccgttagcgt tgaagaactg 2281 ctgcaatatg ttgaactgca agatccggtt acccgtaccc agctgcgtgc aatggcagca 2341 aaaaccgttt gtccgcctca taaagttgaa ctggaagcac tgctggagaa acaggcatat 2401 aaagaacagg ttctggcaaa acgtctgacc atgctggaac tgctggaaaa atatccggca 2461 tgcgaaatga aattcagcga atttattgca ctgctgccga gtattcgtcc gcgttattat 2521 agcattagca gcagtccgcg tgttgatgaa aaacaggcca gcattaccgt tagcgtggtt 2581 agcggtgaag catggtcagg ttatggtgaa tataaaggta ttgccagcaa ttatctggcc 2641 gaactgcaag aaggtgatac cattacctgt tttattagca caccgcagag cgaatttacc 2701 ctgccgaaag atcctgaaac accgctgatt atggttggtc cgggtacagg tgttgcaccg 2761 tttcgtggtt ttgttcaggc acgtaaacaa ctgaaagaac agggtcagag cctgggtgaa 2821 gcacatctgt attttggttg tcgtagtccg catgaggatt atctgtatca agaagaactg 2881 gaaaacgcac agagcgaagg tattattacc ctgcataccg catttagccg tatgccgaat 2941 cagccgaaaa catatgttca gcatgttatg gaacaggatg gcaaaaaact gattgaactg 3001 ctggatcagg gtgcccattt ctatatttgt ggtgatggta gccagatggc accggcagtt 3061 gaagcaaccc tgatgaaaag ctatgcagat gttcatcagg ttagcgaagc agatgcacgt 3121 ctgtggctgc aacagctgga agaaaaaggt cgttatgcaa aagatgtttg ggcaggttaa 3181 taatactagt agcggccgct gcag //