##gff-version 3
##sequence-region BBa_J100100 1 3204
BBa_J100100	.	non_covalent_binding_site	23	28	.	+	0	ID=annotation2211531;Name=BsaI cuts right
BBa_J100100	.	sequence_feature	30	33	.	+	0	ID=annotation2211532;Name=BsaI sticky end
BBa_J100100	.	start_codon	31	33	.	+	0	ID=annotation2211529;Name=start
BBa_J100100	.	engineered_region	3184	3204	.	+	0	ID=annotation2211528;Name=Bba_suffix
BBa_J100100	.	stop_codon	3178	3183	.	+	0	ID=annotation2211530;Name=Stop
BBa_J100100	.	engineered_region	1	22	.	+	0	ID=annotation2211527;Name=Bba_prefix
>BBa_J100100
gaattcgcggccgcttctagagggtctcgaatgaccatcaaagaaatgccgcagccgaaaacctttggtgaactgaaaa
atctgccgctgctgaataccgataaaccggttcatgcactgatgaaaattgcagatgaactgggcgagatctttaaatt
cgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgattaaagaagcatgcgacgaaagccgctttgat
aaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatggtctgagcaccagctggacccatgaaaaaa
actggaaaaaagcccataatattctgctgccgagctttagccagcaggcaatgaaaggttatcatgcaatgatggttga
tattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaacatattgaagttccggaagatatgacccgt
ctgaccctggataccattggtctgtgtggttttaactatcgcttcaacagcttttatcgtgatcagccgcatccgtttg
ttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcgtgcaaatccggatgatccggcatatgatga
aaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtggacaaaattatcgcagatcgtaaagcaagc
ggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatccggaaaccggtgaaccgctggatgatgaaa
acattcgttatcagattattaccttcctgattgccggtcatgaaaccaccagtggtctgctgagctttgcactgtattt
tctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagcacgcgttctggttgatccggttccgagctat
aaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcactgcgtctgtggccgacctttccgtggttta
gcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctggaaaaaggtgatgaactgatggttctgattcc
gcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaatttcgtccggaacgttttgaaaatccgagcgca
attccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgtattggtcagcagtttgcactgcatgaagcca
ccctggttctgggtatgatgctgaaacattttgattttgaggaccacaccaactatgagctggatattaaaggcaccct
gaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatcccgctgggtggtattccgagcccgagcacc
gaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataatacaccgctgctggttctgtatggtagcaata
tgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgagcaaaggttttgcaccgcaggttgcaaccct
ggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgttaccgcaagctataatggtcatccgcctgat
aatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaagttaaaggtgttcgttatagcgtttttggtt
gcggtgataaaaactgggcaaccacctatcagaaagttccggcatttattgatgaaaccctggcagcaaaaggtgcaga
aaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggcacctatgaagaatggcgtgaacatatgtgg
tcagatgttgcagcctattttaacctggatatcgaaaacagcgaggacaataaaagcaccctgagcctgcaatttgtgg
atagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcaccaatgttgttgcaagcaaagaactgcaaca
gcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaagaagcaagctatcaagagggtgatcatctg
ggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcacgttttggtctggatgcaagccagcagattc
gtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaaccgttagcgttgaagaactgctgcaatatgt
tgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagcaaaaaccgtttgtccgcctcataaagttgaa
ctggaagcactgctggagaaacaggcatataaagaacaggttctggcaaaacgtctgaccatgctggaactgctggaaa
aatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgccgagtattcgtccgcgttattatagcattag
cagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtggttagcggtgaagcatggtcaggttatggt
gaatataaaggtattgccagcaattatctggccgaactgcaagaaggtgataccattacctgttttattagcacaccgc
agagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggttggtccgggtacaggtgttgcaccgtttcg
tggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctgggtgaagcacatctgtattttggttgtcgt
agtccgcatgaggattatctgtatcaagaagaactggaaaacgcacagagcgaaggtattattaccctgcataccgcat
ttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatggaacaggatggcaaaaaactgattgaactgct
ggatcagggtgcccatttctatatttgtggtgatggtagccagatggcaccggcagttgaagcaaccctgatgaaaagc
tatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgcaacagctggaagaaaaaggtcgttatgcaa
aagatgtttgggcaggttaataatactagtagcggccgctgcag