LOCUS       BBa_J100101             3295 bp    DNA     linear       22-Dec-2024
DEFINITION  High production of eCDM8
ACCESSION   BBa_J100101
VERSION     BBa_J100101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..36
                     /label=BBa_J119031
     misc_feature    37..124
                     /label=BBa_J119025
     RBS             55..63
                     /label=leader RBS
     misc_feature    69..71
                     /label=Start
     misc_feature    107..115
                     /label=GOI RBS
     misc_feature    120..122
                     /label=Stop
     misc_feature    122..124
                     /label=Start
     misc_feature    125..3328
                     /label=BBa_J100100
     misc_feature    121..124
                     /label=BsaI sticky end
     misc_feature    3302..3307
                     /label=Stop
     misc_feature    3275..3295
                     /label=Bba_suffix
     misc_feature    3269..3274
                     /label=Double Stop
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggagggc ccaagttcac ttaaaaagga
       61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gaggttttct
      121 aatgaccatc aaagaaatgc cgcagccgaa aacctttggt gaactgaaaa atctgccgct
      181 gctgaatacc gataaaccgg ttcatgcact gatgaaaatt gcagatgaac tgggcgagat
      241 ctttaaattc gaagcaccgg gtagcgttac ccgttatctg agcagccagc gtctgattaa
      301 agaagcatgc gacgaaagcc gctttgataa aaatctgatt cagtggctga aattcatccg
      361 cgattttctg ggtgatggtc tgagcaccag ctggacccat gaaaaaaact ggaaaaaagc
      421 ccataatatt ctgctgccga gctttagcca gcaggcaatg aaaggttatc atgcaatgat
      481 ggttgatatt gccgttcagc tggttcagaa atgggaacgt ctgaatgcag atgaacatat
      541 tgaagttccg gaagatatga cccgtctgac cctggatacc attggtctgt gtggttttaa
      601 ctatcgcttc aacagctttt atcgtgatca gccgcatccg tttgttacca gcatggttcg
      661 tgcactggat gaagcagtga ataaatggca gcgtgcaaat ccggatgatc cggcatatga
      721 tgaaaataaa cgtcagttcc aagaggatat taaagtgatg aatgacctgg tggacaaaat
      781 tatcgcagat cgtaaagcaa gcggtgaaca gagtgatgat ctgctgaccc atatgctgaa
      841 tggtaaagat ccggaaaccg gtgaaccgct ggatgatgaa aacattcgtt atcagattat
      901 taccttcctg attgccggtc atgaaaccac cagtggtctg ctgagctttg cactgtattt
      961 tctggttaaa aatccgcacg ttctgcaaaa agcagccgaa gaagcagcac gcgttctggt
     1021 tgatccggtt ccgagctata aacaggttaa acagctgaaa tatgtgggca tggttctgaa
     1081 tgaagcactg cgtctgtggc cgacctttcc gtggtttagc ctgtatgcaa aagaagatac
     1141 cgttctggga ggtgaatatc cgctggaaaa aggtgatgaa ctgatggttc tgattccgca
     1201 gctgcatcgt gataaaacca tttggggtga tgatgtggaa gaatttcgtc cggaacgttt
     1261 tgaaaatccg agcgcaattc cgcagcatgc ctttaaaccg tttggtaatg gtcagcgtgc
     1321 atgtattggt cagcagtttg cactgcatga agccaccctg gttctgggta tgatgctgaa
     1381 acattttgat tttgaggacc acaccaacta tgagctggat attaaaggca ccctgaccct
     1441 gaaaccggaa ggttttgttg ttaaagccaa aagcaaaaaa atcccgctgg gtggtattcc
     1501 gagcccgagc accgaacaga gcgcaaaaaa agttcgtaaa aaagccgaaa acgcccataa
     1561 tacaccgctg ctggttctgt atggtagcaa tatgggtaca gccgaaggca ccgcacgtga
     1621 tctggcagat attgcaatga gcaaaggttt tgcaccgcag gttgcaaccc tggatagcca
     1681 tgcaggtaat ctgcctcgtg aaggtgcagt tctgattgtt accgcaagct ataatggtca
     1741 tccgcctgat aatgcaaaac agtttgttga ttggctggat caggcaagcg cagatgaagt
     1801 taaaggtgtt cgttatagcg tttttggttg cggtgataaa aactgggcaa ccacctatca
     1861 gaaagttccg gcatttattg atgaaaccct ggcagcaaaa ggtgcagaaa atattgcaga
     1921 tcgtggtgaa accgatgcca gtgatgattt tgaaggcacc tatgaagaat ggcgtgaaca
     1981 tatgtggtca gatgttgcag cctattttaa cctggatatc gaaaacagcg aggacaataa
     2041 aagcaccctg agcctgcaat ttgtggatag cgcagcagat atgccgctgg caaaaatgca
     2101 tggtgcattt agcaccaatg ttgttgcaag caaagaactg caacagcctg gtagcgcacg
     2161 tagcacccgt catctggaaa ttgaactgcc gaaagaagca agctatcaag agggtgatca
     2221 tctgggtgtt attccgcgta attatgaagg tattgttaat cgtgttaccg cacgttttgg
     2281 tctggatgca agccagcaga ttcgtctgga agcagaagaa gaaaaactgg cacatctgcc
     2341 gctggccaaa accgttagcg ttgaagaact gctgcaatat gttgaactgc aagatccggt
     2401 tacccgtacc cagctgcgtg caatggcagc aaaaaccgtt tgtccgcctc ataaagttga
     2461 actggaagca ctgctggaga aacaggcata taaagaacag gttctggcaa aacgtctgac
     2521 catgctggaa ctgctggaaa aatatccggc atgcgaaatg aaattcagcg aatttattgc
     2581 actgctgccg agtattcgtc cgcgttatta tagcattagc agcagtccgc gtgttgatga
     2641 aaaacaggcc agcattaccg ttagcgtggt tagcggtgaa gcatggtcag gttatggtga
     2701 atataaaggt attgccagca attatctggc cgaactgcaa gaaggtgata ccattacctg
     2761 ttttattagc acaccgcaga gcgaatttac cctgccgaaa gatcctgaaa caccgctgat
     2821 tatggttggt ccgggtacag gtgttgcacc gtttcgtggt tttgttcagg cacgtaaaca
     2881 actgaaagaa cagggtcaga gcctgggtga agcacatctg tattttggtt gtcgtagtcc
     2941 gcatgaggat tatctgtatc aagaagaact ggaaaacgca cagagcgaag gtattattac
     3001 cctgcatacc gcatttagcc gtatgccgaa tcagccgaaa acatatgttc agcatgttat
     3061 ggaacaggat ggcaaaaaac tgattgaact gctggatcag ggtgcccatt tctatatttg
     3121 tggtgatggt agccagatgg caccggcagt tgaagcaacc ctgatgaaaa gctatgcaga
     3181 tgttcatcag gttagcgaag cagatgcacg tctgtggctg caacagctgg aagaaaaagg
     3241 tcgttatgca aaagatgttt gggcaggtta ataatactag tagcggccgc tgcag
//