##gff-version 3
##sequence-region BBa_J100101 1 3328
BBa_J100101	.	stop_codon	3302	3307	.	+	0	ID=annotation2214167;Name=Stop
BBa_J100101	.	engineered_region	37	124	.	+	0	ID=annotation2214159;Name=BBa_J119025
BBa_J100101	.	engineered_region	125	3328	.	+	0	ID=annotation2214165;Name=BBa_J100100
BBa_J100101	.	sequence_feature	107	115	.	+	0	ID=annotation2214162;Name=GOI RBS
BBa_J100101	.	engineered_region	3275	3295	.	+	0	ID=annotation2214168;Name=Bba_suffix
BBa_J100101	.	sequence_feature	121	124	.	+	0	ID=annotation2214166;Name=BsaI sticky end
BBa_J100101	.	engineered_region	1	36	.	+	0	ID=annotation2214158;Name=BBa_J119031
BBa_J100101	.	start_codon	69	71	.	+	0	ID=annotation2214161;Name=Start
BBa_J100101	.	stop_codon	3269	3274	.	+	0	ID=annotation2214169;Name=Double Stop
BBa_J100101	.	stop_codon	120	122	.	+	0	ID=annotation2214163;Name=Stop
BBa_J100101	.	start_codon	122	124	.	+	0	ID=annotation2214164;Name=Start
BBa_J100101	.	ribosome_entry_site	55	63	.	+	0	ID=annotation2214160;Name=leader RBS
>BBa_J100101
ttgacaattaatcatccggctcgtaatttatgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaat
tttcgtactgaaacatcttaatcatgctaaggaggttttctaatgaccatcaaagaaatgccgcagccgaaaacctttg
gtgaactgaaaaatctgccgctgctgaataccgataaaccggttcatgcactgatgaaaattgcagatgaactgggcga
gatctttaaattcgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgattaaagaagcatgcgacgaa
agccgctttgataaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatggtctgagcaccagctgga
cccatgaaaaaaactggaaaaaagcccataatattctgctgccgagctttagccagcaggcaatgaaaggttatcatgc
aatgatggttgatattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaacatattgaagttccggaa
gatatgacccgtctgaccctggataccattggtctgtgtggttttaactatcgcttcaacagcttttatcgtgatcagc
cgcatccgtttgttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcgtgcaaatccggatgatcc
ggcatatgatgaaaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtggacaaaattatcgcagat
cgtaaagcaagcggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatccggaaaccggtgaaccgc
tggatgatgaaaacattcgttatcagattattaccttcctgattgccggtcatgaaaccaccagtggtctgctgagctt
tgcactgtattttctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagcacgcgttctggttgatccg
gttccgagctataaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcactgcgtctgtggccgacct
ttccgtggtttagcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctggaaaaaggtgatgaactgat
ggttctgattccgcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaatttcgtccggaacgttttgaa
aatccgagcgcaattccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgtattggtcagcagtttgcac
tgcatgaagccaccctggttctgggtatgatgctgaaacattttgattttgaggaccacaccaactatgagctggatat
taaaggcaccctgaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatcccgctgggtggtattccg
agcccgagcaccgaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataatacaccgctgctggttctgt
atggtagcaatatgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgagcaaaggttttgcaccgca
ggttgcaaccctggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgttaccgcaagctataatggt
catccgcctgataatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaagttaaaggtgttcgttata
gcgtttttggttgcggtgataaaaactgggcaaccacctatcagaaagttccggcatttattgatgaaaccctggcagc
aaaaggtgcagaaaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggcacctatgaagaatggcgt
gaacatatgtggtcagatgttgcagcctattttaacctggatatcgaaaacagcgaggacaataaaagcaccctgagcc
tgcaatttgtggatagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcaccaatgttgttgcaagcaa
agaactgcaacagcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaagaagcaagctatcaagag
ggtgatcatctgggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcacgttttggtctggatgcaa
gccagcagattcgtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaaccgttagcgttgaagaact
gctgcaatatgttgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagcaaaaaccgtttgtccgcct
cataaagttgaactggaagcactgctggagaaacaggcatataaagaacaggttctggcaaaacgtctgaccatgctgg
aactgctggaaaaatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgccgagtattcgtccgcgtta
ttatagcattagcagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtggttagcggtgaagcatgg
tcaggttatggtgaatataaaggtattgccagcaattatctggccgaactgcaagaaggtgataccattacctgtttta
ttagcacaccgcagagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggttggtccgggtacaggtgt
tgcaccgtttcgtggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctgggtgaagcacatctgtat
tttggttgtcgtagtccgcatgaggattatctgtatcaagaagaactggaaaacgcacagagcgaaggtattattaccc
tgcataccgcatttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatggaacaggatggcaaaaaact
gattgaactgctggatcagggtgcccatttctatatttgtggtgatggtagccagatggcaccggcagttgaagcaacc
ctgatgaaaagctatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgcaacagctggaagaaaaag
gtcgttatgcaaaagatgtttgggcaggttaataatactagtagcggccgctgcag