LOCUS       BBa_J100104             1631 bp    DNA     linear       23-Dec-2024
DEFINITION  For Testing New Promoters via Golden Gate Assembly v2
ACCESSION   BBa_J100104
VERSION     BBa_J100104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa Prefix
     misc_feature    23..26
                     /label=right sticky end
     misc_feature    28..33
                     /label=BsaI cuts left
     CDS             34..833
                     /label=J100103 rev
     misc_feature    834..839
                     /label=BsaI cuts right
     misc_feature    841..844
                     /label=left sticky end
     RBS             845..929
                     /label=BD18
     CDS             930..1610
                     /label=mRFP
     misc_feature    1605..1610
                     /label=double stop
     misc_feature    34..39
                     /label=double stop
     misc_feature    1611..1631
                     /label=BBa suffix
     CDS             34..702
                     /label=blue protein
     RBS             703..787
                     /label=BD18 reverse
     promoter        788..833
                     /label=P2 reverse
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag accttattag gcaacaaccg gtttacgtgc
       61 aatgctaatt tcgcactgtt caacgctggt gtaatcttta ttgtggttgg tcacatccag
      121 tttacgatcc acataatgat aaccaggcat tttcaccggt tttttggctt tgtaggtggt
      181 tttaaactca cacagataat gaccaccacc ttccagtttc agtgccataa agttattacc
      241 cagcagcatg ccatcacgtg caaacagacg ttcggtattc ggttcccaac cctgggtttt
      301 tttctgcata accggaccat taggcggaaa attcagaccg ctaaatttca cgtggtagat
      361 aaagcaatta ccctgaatgc tgctatcatt gctaacggtg caaactgcac catcttcaaa
      421 gttcataata cgttcccagg tataaccttc cggaaagctc tgtttcacat aatccgggat
      481 atcttccgga tatttggtaa acggaatgct accatactga cactgcggac tcagaatatc
      541 ccatgcaaac ggcagcggac cacctttggt aacggtcagt ttaacggtct gttcgccttc
      601 atacggtttg cctttaccat caccttcaac ttcaaaataa tggccgttca cggtgccgct
      661 catatacact ttataggtca tctgctttgc aatcacgctc attagaaacg ctccgtcgca
      721 tgattaagat gtttcagtac gaaaattgct ttcattgttg atctcctttt taagtgaact
      781 tgggccctcc acacattata ggtacaaaaa gatgcgaagt caatactctt tttggtctca
      841 gcgggggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa
      901 catcttaatc atgcgacgga gcgtttctaa tggcttcctc cgaagatgtt atcaaagagt
      961 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     1021 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     1081 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     1141 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     1201 tcaaatggga acgtgttatg aacttcgaag atggtggtgt tgttaccgtt acccaggact
     1261 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     1321 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     1381 acccggaaga tggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     1441 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     1501 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagat tacaccatcg
     1561 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa tactagtagc
     1621 ggccgctgca g
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