##gff-version 3
##sequence-region BBa_J100104 1 1631
BBa_J100104	.	promoter	788	833	.	+	0	ID=annotation2213794;Name=P2 reverse
BBa_J100104	.	engineered_region	1611	1631	.	+	0	ID=annotation2213791;Name=BBa suffix
BBa_J100104	.	sequence_feature	23	26	.	+	0	ID=annotation2213782;Name=right sticky end
BBa_J100104	.	CDS	34	833	.	+	0	ID=annotation2213784;Name=J100103 rev
BBa_J100104	.	non_covalent_binding_site	28	33	.	+	0	ID=annotation2213783;Name=BsaI cuts left
BBa_J100104	.	stop_codon	1605	1610	.	+	0	ID=annotation2213789;Name=double stop
BBa_J100104	.	stop_codon	34	39	.	+	0	ID=annotation2213790;Name=double stop
BBa_J100104	.	CDS	34	702	.	+	0	ID=annotation2213792;Name=blue protein
BBa_J100104	.	CDS	930	1610	.	+	0	ID=annotation2213788;Name=mRFP
BBa_J100104	.	engineered_region	1	22	.	+	0	ID=annotation2213781;Name=BBa Prefix
BBa_J100104	.	ribosome_entry_site	703	787	.	+	0	ID=annotation2213793;Name=BD18 reverse
BBa_J100104	.	ribosome_entry_site	845	929	.	+	0	ID=annotation2213787;Name=BD18
BBa_J100104	.	sequence_feature	841	844	.	+	0	ID=annotation2213786;Name=left sticky end
BBa_J100104	.	sequence_feature	834	839	.	+	0	ID=annotation2213785;Name=BsaI cuts right
>BBa_J100104
gaattcgcggccgcttctagagcgactgagaccttattaggcaacaaccggtttacgtgcaatgctaatttcgcactgt
tcaacgctggtgtaatctttattgtggttggtcacatccagtttacgatccacataatgataaccaggcattttcaccg
gttttttggctttgtaggtggttttaaactcacacagataatgaccaccaccttccagtttcagtgccataaagttatt
acccagcagcatgccatcacgtgcaaacagacgttcggtattcggttcccaaccctgggtttttttctgcataaccgga
ccattaggcggaaaattcagaccgctaaatttcacgtggtagataaagcaattaccctgaatgctgctatcattgctaa
cggtgcaaactgcaccatcttcaaagttcataatacgttcccaggtataaccttccggaaagctctgtttcacataatc
cgggatatcttccggatatttggtaaacggaatgctaccatactgacactgcggactcagaatatcccatgcaaacggc
agcggaccacctttggtaacggtcagtttaacggtctgttcgccttcatacggtttgcctttaccatcaccttcaactt
caaaataatggccgttcacggtgccgctcatatacactttataggtcatctgctttgcaatcacgctcattagaaacgc
tccgtcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccctcc
acacattataggtacaaaaagatgcgaagtcaatactctttttggtctcagcgggggcccaagttcacttaaaaaggag
atcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcag