LOCUS BBa_J100107 2699 bp DNA linear 22-Nov-2024 DEFINITION E. coli adhE gene ACCESSION BBa_J100107 VERSION BBa_J100107.1 FEATURES Location/Qualifiers misc_feature 2..5 /label=BsaI sticky end misc_feature 3..5 /label=start codon misc_feature 2676..2678 /label=stop codon CDS 3..2678 /label=ORF adhE ORIGIN 1 gaatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc 61 agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc 121 tggctgctgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg 181 gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct 241 ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta 301 tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg 361 ctatcttcaa atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc 421 cgcgtgcaaa agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg 481 ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg 541 cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta 601 aagccgcata cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg 661 ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct 721 tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg 781 acgctgtacg tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga 841 aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc 901 cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga 961 tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga 1021 ctctggcaat gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg 1081 ttgctatggg cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg 1141 ctcgcgtttc ttacttcggt cagaaaatga aaacggcgcg tatcctgatt aacaccccag 1201 cgtctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg 1261 gttgtggttc ttggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca 1321 acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta 1381 tctacttccg ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca 1441 aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca 1501 cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc 1561 cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga 1621 ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg 1681 aacatccgga aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta 1741 tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg 1801 gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat 1861 atccgctggc agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta 1921 tggacatgcc gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg 1981 aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga 2041 aactgctgaa agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc 2101 gtgaacgtgt tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg 2161 gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg 2221 caaacgccct gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc 2281 agactgcatt cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg 2341 accacttggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg 2401 catggctgga aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg 2461 ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc 2521 agtgcaccgg cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg 2581 atacctacta cggtcgtgat tatgtagaag gtgaaactgc agcgaagaaa gaagctgctc 2641 cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaata ctagtagcgg ccgctgcag //