LOCUS BBa_J100108 4182 bp DNA linear 29-Nov-2024 DEFINITION Riboswitch and GFP +High production of eCDM8 ACCESSION BBa_J100108 VERSION BBa_J100108.1 FEATURES Location/Qualifiers promoter 1..53 /label=T5 promoter misc_feature 54..74 /label=lac operator misc_feature 75..80 /label=scar misc_feature 97..102 /label=KpnI site misc_feature 82..96 /label=constant sequence misc_feature 102..162 /label=Riboswitch RBS 146..149 /label=RBS misc_feature 160..162 /label=Start CDS 160..879 /label=superfolder GFP misc_feature 1..879 /label=BBa_J100079 misc_feature 888..923 /label=BBa_J119031 misc_feature 924..1011 /label=BBa_J119025 RBS 942..950 /label=leader RBS misc_feature 956..958 /label=Start misc_feature 994..1002 /label=GOI RBS misc_feature 1007..1009 /label=Stop misc_feature 1009..1011 /label=Start misc_feature 1012..4215 /label=BBa_J100100 misc_feature 1008..1011 /label=BsaI sticky end misc_feature 4189..4194 /label=Stop misc_feature 4162..4182 /label=Bba_suffix misc_feature 4156..4161 /label=Double Stop misc_feature 888..4182 /label=BBa_J100101 ORIGIN 1 aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg 61 agcggataac aattactaga gatacgactc actataggta ccggtgatac cagcatcgtc 121 ttgatgccct tggcagcacc ctgctaaggt aacaacaaga tgcgtaaagg cgaagagctg 181 ttcaccggtg ttgttccgat tctggttgaa ctggatggtg atgttaatgg ccacaaattt 241 tcagttcgtg gtgaaggcga gggtgatgca accaatggta aactgaccct gaaatttatc 301 tgtaccaccg gcaaactgcc ggttccgtgg ccgaccctgg ttaccaccct gacctatggt 361 gttcagtgtt ttgcacgtta tccggatcat atgaaacagc acgatttttt caaaagcgca 421 atgccggaag gttatgttca agaacgtacc atctccttta aagatgatgg cacctataaa 481 acccgtgccg aagttaaatt tgaaggtgac accctggtga atcgtattga gctgaaaggc 541 atcgatttca aagaggatgg taatatcctg ggccataaac tggaatataa cttcaatagc 601 cacaatgtgt atatcaccgc agacaaacag aaaaacggca ttaaagccaa ctttaagatt 661 cgccataatg tggaagatgg tagcgtgcag ctggcagatc attatcagca gaataccccg 721 attggtgatg gtccggttct gctgccggat aatcactatc tgagcaccca gagcgttctg 781 agcaaagatc cgaatgaaaa acgtgatcac atggtgctgc tggaatttgt taccgcagca 841 ggtattaccc atggtatgga tgaactgtac aaatgatgat actagagttg acaattaatc 901 atccggctcg taatttatgt ggagggccca agttcactta aaaaggagat caacaatgaa 961 agcaattttc gtactgaaac atcttaatca tgctaaggag gttttctaat gaccatcaaa 1021 gaaatgccgc agccgaaaac ctttggtgaa ctgaaaaatc tgccgctgct gaataccgat 1081 aaaccggttc atgcactgat gaaaattgca gatgaactgg gcgagatctt taaattcgaa 1141 gcaccgggta gcgttacccg ttatctgagc agccagcgtc tgattaaaga agcatgcgac 1201 gaaagccgct ttgataaaaa tctgattcag tggctgaaat tcatccgcga ttttctgggt 1261 gatggtctga gcaccagctg gacccatgaa aaaaactgga aaaaagccca taatattctg 1321 ctgccgagct ttagccagca ggcaatgaaa ggttatcatg caatgatggt tgatattgcc 1381 gttcagctgg ttcagaaatg ggaacgtctg aatgcagatg aacatattga agttccggaa 1441 gatatgaccc gtctgaccct ggataccatt ggtctgtgtg gttttaacta tcgcttcaac 1501 agcttttatc gtgatcagcc gcatccgttt gttaccagca tggttcgtgc actggatgaa 1561 gcagtgaata aatggcagcg tgcaaatccg gatgatccgg catatgatga aaataaacgt 1621 cagttccaag aggatattaa agtgatgaat gacctggtgg acaaaattat cgcagatcgt 1681 aaagcaagcg gtgaacagag tgatgatctg ctgacccata tgctgaatgg taaagatccg 1741 gaaaccggtg aaccgctgga tgatgaaaac attcgttatc agattattac cttcctgatt 1801 gccggtcatg aaaccaccag tggtctgctg agctttgcac tgtattttct ggttaaaaat 1861 ccgcacgttc tgcaaaaagc agccgaagaa gcagcacgcg ttctggttga tccggttccg 1921 agctataaac aggttaaaca gctgaaatat gtgggcatgg ttctgaatga agcactgcgt 1981 ctgtggccga cctttccgtg gtttagcctg tatgcaaaag aagataccgt tctgggaggt 2041 gaatatccgc tggaaaaagg tgatgaactg atggttctga ttccgcagct gcatcgtgat 2101 aaaaccattt ggggtgatga tgtggaagaa tttcgtccgg aacgttttga aaatccgagc 2161 gcaattccgc agcatgcctt taaaccgttt ggtaatggtc agcgtgcatg tattggtcag 2221 cagtttgcac tgcatgaagc caccctggtt ctgggtatga tgctgaaaca ttttgatttt 2281 gaggaccaca ccaactatga gctggatatt aaaggcaccc tgaccctgaa accggaaggt 2341 tttgttgtta aagccaaaag caaaaaaatc ccgctgggtg gtattccgag cccgagcacc 2401 gaacagagcg caaaaaaagt tcgtaaaaaa gccgaaaacg cccataatac accgctgctg 2461 gttctgtatg gtagcaatat gggtacagcc gaaggcaccg cacgtgatct ggcagatatt 2521 gcaatgagca aaggttttgc accgcaggtt gcaaccctgg atagccatgc aggtaatctg 2581 cctcgtgaag gtgcagttct gattgttacc gcaagctata atggtcatcc gcctgataat 2641 gcaaaacagt ttgttgattg gctggatcag gcaagcgcag atgaagttaa aggtgttcgt 2701 tatagcgttt ttggttgcgg tgataaaaac tgggcaacca cctatcagaa agttccggca 2761 tttattgatg aaaccctggc agcaaaaggt gcagaaaata ttgcagatcg tggtgaaacc 2821 gatgccagtg atgattttga aggcacctat gaagaatggc gtgaacatat gtggtcagat 2881 gttgcagcct attttaacct ggatatcgaa aacagcgagg acaataaaag caccctgagc 2941 ctgcaatttg tggatagcgc agcagatatg ccgctggcaa aaatgcatgg tgcatttagc 3001 accaatgttg ttgcaagcaa agaactgcaa cagcctggta gcgcacgtag cacccgtcat 3061 ctggaaattg aactgccgaa agaagcaagc tatcaagagg gtgatcatct gggtgttatt 3121 ccgcgtaatt atgaaggtat tgttaatcgt gttaccgcac gttttggtct ggatgcaagc 3181 cagcagattc gtctggaagc agaagaagaa aaactggcac atctgccgct ggccaaaacc 3241 gttagcgttg aagaactgct gcaatatgtt gaactgcaag atccggttac ccgtacccag 3301 ctgcgtgcaa tggcagcaaa aaccgtttgt ccgcctcata aagttgaact ggaagcactg 3361 ctggagaaac aggcatataa agaacaggtt ctggcaaaac gtctgaccat gctggaactg 3421 ctggaaaaat atccggcatg cgaaatgaaa ttcagcgaat ttattgcact gctgccgagt 3481 attcgtccgc gttattatag cattagcagc agtccgcgtg ttgatgaaaa acaggccagc 3541 attaccgtta gcgtggttag cggtgaagca tggtcaggtt atggtgaata taaaggtatt 3601 gccagcaatt atctggccga actgcaagaa ggtgatacca ttacctgttt tattagcaca 3661 ccgcagagcg aatttaccct gccgaaagat cctgaaacac cgctgattat ggttggtccg 3721 ggtacaggtg ttgcaccgtt tcgtggtttt gttcaggcac gtaaacaact gaaagaacag 3781 ggtcagagcc tgggtgaagc acatctgtat tttggttgtc gtagtccgca tgaggattat 3841 ctgtatcaag aagaactgga aaacgcacag agcgaaggta ttattaccct gcataccgca 3901 tttagccgta tgccgaatca gccgaaaaca tatgttcagc atgttatgga acaggatggc 3961 aaaaaactga ttgaactgct ggatcagggt gcccatttct atatttgtgg tgatggtagc 4021 cagatggcac cggcagttga agcaaccctg atgaaaagct atgcagatgt tcatcaggtt 4081 agcgaagcag atgcacgtct gtggctgcaa cagctggaag aaaaaggtcg ttatgcaaaa 4141 gatgtttggg caggttaata atactagtag cggccgctgc ag //