##gff-version 3
##sequence-region BBa_J100108 1 4182
BBa_J100108	.	engineered_region	1	879	.	+	0	ID=BBa_J100079;Name=BBa_J100079
BBa_J100108	.	sequence_feature	97	102	.	+	0	ID=annotation2177536;Name=KpnI site;Parent=BBa_J100079
BBa_J100108	.	sequence_feature	102	162	.	+	0	ID=annotation2177538;Name=Riboswitch;Parent=BBa_J100079
BBa_J100108	.	CDS	160	879	.	+	0	ID=annotation2177541;Name=superfolder GFP;Parent=BBa_J100079
BBa_J100108	.	ribosome_entry_site	146	149	.	+	0	ID=annotation2177539;Name=RBS;Parent=BBa_J100079
BBa_J100108	.	sequence_feature	75	80	.	+	0	ID=annotation2177535;Name=scar;Parent=BBa_J100079
BBa_J100108	.	operator	54	74	.	+	0	ID=annotation2177534;Name=lac operator ;Parent=BBa_J100079
BBa_J100108	.	start_codon	160	162	.	+	0	ID=annotation2177540;Name=Start;Parent=BBa_J100079
BBa_J100108	.	promoter	1	53	.	+	0	ID=annotation2177533;Name=T5 promoter;Parent=BBa_J100079
BBa_J100108	.	sequence_feature	82	96	.	+	0	ID=annotation2177537;Name=constant sequence;Parent=BBa_J100079
BBa_J100108	.	engineered_region	888	4182	.	+	0	ID=BBa_J100101;Name=BBa_J100101
BBa_J100108	.	stop_codon	4189	4194	.	+	0	ID=annotation2214167;Name=Stop;Parent=BBa_J100101
BBa_J100108	.	engineered_region	1012	4215	.	+	0	ID=annotation2214165;Name=BBa_J100100;Parent=BBa_J100101
BBa_J100108	.	engineered_region	924	1011	.	+	0	ID=annotation2214159;Name=BBa_J119025;Parent=BBa_J100101
BBa_J100108	.	start_codon	956	958	.	+	0	ID=annotation2214161;Name=Start;Parent=BBa_J100101
BBa_J100108	.	engineered_region	4162	4182	.	+	0	ID=annotation2214168;Name=Bba_suffix;Parent=BBa_J100101
BBa_J100108	.	ribosome_entry_site	942	950	.	+	0	ID=annotation2214160;Name=leader RBS;Parent=BBa_J100101
BBa_J100108	.	stop_codon	1007	1009	.	+	0	ID=annotation2214163;Name=Stop;Parent=BBa_J100101
BBa_J100108	.	sequence_feature	1008	1011	.	+	0	ID=annotation2214166;Name=BsaI sticky end;Parent=BBa_J100101
BBa_J100108	.	engineered_region	888	923	.	+	0	ID=annotation2214158;Name=BBa_J119031;Parent=BBa_J100101
BBa_J100108	.	start_codon	1009	1011	.	+	0	ID=annotation2214164;Name=Start;Parent=BBa_J100101
BBa_J100108	.	sequence_feature	994	1002	.	+	0	ID=annotation2214162;Name=GOI RBS;Parent=BBa_J100101
BBa_J100108	.	stop_codon	4156	4161	.	+	0	ID=annotation2214169;Name=Double Stop;Parent=BBa_J100101
>BBa_J100108
aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactag
agatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaa
gatgcgtaaaggcgaagagctgttcaccggtgttgttccgattctggttgaactggatggtgatgttaatggccacaaa
ttttcagttcgtggtgaaggcgagggtgatgcaaccaatggtaaactgaccctgaaatttatctgtaccaccggcaaac
tgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgttttgcacgttatccggatcatatgaa
acagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatctcctttaaagatgatggcacc
tataaaacccgtgccgaagttaaatttgaaggtgacaccctggtgaatcgtattgagctgaaaggcatcgatttcaaag
aggatggtaatatcctgggccataaactggaatataacttcaatagccacaatgtgtatatcaccgcagacaaacagaa
aaacggcattaaagccaactttaagattcgccataatgtggaagatggtagcgtgcagctggcagatcattatcagcag
aataccccgattggtgatggtccggttctgctgccggataatcactatctgagcacccagagcgttctgagcaaagatc
cgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccatggtatggatgaactgta
caaatgatgatactagagttgacaattaatcatccggctcgtaatttatgtggagggcccaagttcacttaaaaaggag
atcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgaccatcaaagaaatgc
cgcagccgaaaacctttggtgaactgaaaaatctgccgctgctgaataccgataaaccggttcatgcactgatgaaaat
tgcagatgaactgggcgagatctttaaattcgaagcaccgggtagcgttacccgttatctgagcagccagcgtctgatt
aaagaagcatgcgacgaaagccgctttgataaaaatctgattcagtggctgaaattcatccgcgattttctgggtgatg
gtctgagcaccagctggacccatgaaaaaaactggaaaaaagcccataatattctgctgccgagctttagccagcaggc
aatgaaaggttatcatgcaatgatggttgatattgccgttcagctggttcagaaatgggaacgtctgaatgcagatgaa
catattgaagttccggaagatatgacccgtctgaccctggataccattggtctgtgtggttttaactatcgcttcaaca
gcttttatcgtgatcagccgcatccgtttgttaccagcatggttcgtgcactggatgaagcagtgaataaatggcagcg
tgcaaatccggatgatccggcatatgatgaaaataaacgtcagttccaagaggatattaaagtgatgaatgacctggtg
gacaaaattatcgcagatcgtaaagcaagcggtgaacagagtgatgatctgctgacccatatgctgaatggtaaagatc
cggaaaccggtgaaccgctggatgatgaaaacattcgttatcagattattaccttcctgattgccggtcatgaaaccac
cagtggtctgctgagctttgcactgtattttctggttaaaaatccgcacgttctgcaaaaagcagccgaagaagcagca
cgcgttctggttgatccggttccgagctataaacaggttaaacagctgaaatatgtgggcatggttctgaatgaagcac
tgcgtctgtggccgacctttccgtggtttagcctgtatgcaaaagaagataccgttctgggaggtgaatatccgctgga
aaaaggtgatgaactgatggttctgattccgcagctgcatcgtgataaaaccatttggggtgatgatgtggaagaattt
cgtccggaacgttttgaaaatccgagcgcaattccgcagcatgcctttaaaccgtttggtaatggtcagcgtgcatgta
ttggtcagcagtttgcactgcatgaagccaccctggttctgggtatgatgctgaaacattttgattttgaggaccacac
caactatgagctggatattaaaggcaccctgaccctgaaaccggaaggttttgttgttaaagccaaaagcaaaaaaatc
ccgctgggtggtattccgagcccgagcaccgaacagagcgcaaaaaaagttcgtaaaaaagccgaaaacgcccataata
caccgctgctggttctgtatggtagcaatatgggtacagccgaaggcaccgcacgtgatctggcagatattgcaatgag
caaaggttttgcaccgcaggttgcaaccctggatagccatgcaggtaatctgcctcgtgaaggtgcagttctgattgtt
accgcaagctataatggtcatccgcctgataatgcaaaacagtttgttgattggctggatcaggcaagcgcagatgaag
ttaaaggtgttcgttatagcgtttttggttgcggtgataaaaactgggcaaccacctatcagaaagttccggcatttat
tgatgaaaccctggcagcaaaaggtgcagaaaatattgcagatcgtggtgaaaccgatgccagtgatgattttgaaggc
acctatgaagaatggcgtgaacatatgtggtcagatgttgcagcctattttaacctggatatcgaaaacagcgaggaca
ataaaagcaccctgagcctgcaatttgtggatagcgcagcagatatgccgctggcaaaaatgcatggtgcatttagcac
caatgttgttgcaagcaaagaactgcaacagcctggtagcgcacgtagcacccgtcatctggaaattgaactgccgaaa
gaagcaagctatcaagagggtgatcatctgggtgttattccgcgtaattatgaaggtattgttaatcgtgttaccgcac
gttttggtctggatgcaagccagcagattcgtctggaagcagaagaagaaaaactggcacatctgccgctggccaaaac
cgttagcgttgaagaactgctgcaatatgttgaactgcaagatccggttacccgtacccagctgcgtgcaatggcagca
aaaaccgtttgtccgcctcataaagttgaactggaagcactgctggagaaacaggcatataaagaacaggttctggcaa
aacgtctgaccatgctggaactgctggaaaaatatccggcatgcgaaatgaaattcagcgaatttattgcactgctgcc
gagtattcgtccgcgttattatagcattagcagcagtccgcgtgttgatgaaaaacaggccagcattaccgttagcgtg
gttagcggtgaagcatggtcaggttatggtgaatataaaggtattgccagcaattatctggccgaactgcaagaaggtg
ataccattacctgttttattagcacaccgcagagcgaatttaccctgccgaaagatcctgaaacaccgctgattatggt
tggtccgggtacaggtgttgcaccgtttcgtggttttgttcaggcacgtaaacaactgaaagaacagggtcagagcctg
ggtgaagcacatctgtattttggttgtcgtagtccgcatgaggattatctgtatcaagaagaactggaaaacgcacaga
gcgaaggtattattaccctgcataccgcatttagccgtatgccgaatcagccgaaaacatatgttcagcatgttatgga
acaggatggcaaaaaactgattgaactgctggatcagggtgcccatttctatatttgtggtgatggtagccagatggca
ccggcagttgaagcaaccctgatgaaaagctatgcagatgttcatcaggttagcgaagcagatgcacgtctgtggctgc
aacagctggaagaaaaaggtcgttatgcaaaagatgtttgggcaggttaataatactagtagcggccgctgcag