LOCUS BBa_J100109 2711 bp DNA linear 18-Jun-2024 DEFINITION adhE optimized for E. coli, PstI removed ACCESSION BBa_J100109 VERSION BBa_J100109.1 FEATURES Location/Qualifiers misc_feature 2692..2711 /label=suffix misc_feature 1..6 /label=EcoRI site misc_feature 7..12 /label=BsaI cuts right misc_feature 14..17 /label=BsaI sticky end misc_feature 15..17 /label=start CDS 15..2687 /label=adhE allele misc_feature 2688..2690 /label=stop ORIGIN 1 gaattcggtc tcgaatggca gttaccaatg ttgcagaact gaatgcactg gttgaacgtg 61 ttaaaaaagc acagcgtgaa tatgcaagct ttacccaaga acaggtggac aaaatctttc 121 gtgcagcagc actggcagca gccgatgcac gtattccgct ggcaaaaatg gccgttgccg 181 aaagcggtat gggtattgtt gaagataaag tgatcaaaaa ccacttcgcc agcgagtata 241 tctataacgc ctataaagat gagaaaacct gtggtgttct gagcgaagat gatacctttg 301 gcaccattac cattgccgaa ccgattggca ttatttgtgg tattgtgccg accaccaatc 361 cgaccagcac cgcaatcttt aaaagcctga ttagcctgaa aacccgcaac gcaattatct 421 ttagtccgca tccgcgtgca aaagatgcaa ccaataaagc agcagatatt gtgctgcaag 481 cagcaattgc agcgggtgct ccgaaagatc tgattggttg gattgatcag ccgagcgttg 541 aactgagcaa tgccctgatg caccatccgg atattaatct gattctggca accggtggtc 601 cgggtatggt taaagccgca tatagcagcg gtaaaccggc aattggtgtt ggtgcaggta 661 atactccggt tgttattgat gaaaccgctg atattaaacg tgcagttgca agcgttctga 721 tgagcaaaac atttgataat ggtgttattt gcgcaagcga acagagcgtt gttgttgtgg 781 atagcgttta tgatgcagtt cgtgaacgtt ttgcaaccca tggtggttac ctgttacagg 841 gtaaagagct gaaagcagtt caggatgtga ttctgaaaaa tggtgcgctg aatgccgcaa 901 ttgttggtca gcctgcgtat aaaatcgccg aactggcagg ttttagcgtt ccggaaaaca 961 ccaaaattct gatcggtgaa gttaccgtgg tggatgaaag cgaaccgttt gcacatgaaa 1021 aactgagccc gaccctggca atgtatcgtg cgaaagactt tgaagatgcc gttgaaaaag 1081 cagagaaact ggttgcaatg ggtggcattg gtcataccag ctgtctgtat acggatcagg 1141 ataatcaacc ggcacgtgtt agctattttg gccagaaaat gaaaacagca cgcattctga 1201 ttaatacacc ggcaagccag ggtggtatcg gtgatctgta taactttaaa ctggcaccga 1261 gtctgaccct gggttgtggt agctggggtg gtaatagcat tagcgaaaat gttggtccga 1321 aacacctgat caacaaaaaa accgttgcca aacgtgccga aaatatgctg tggcataaac 1381 tgcctaaaag catctatttt cgtcgtggta gcctgccgat tgccctggat gaagttatta 1441 ccgatggtca caaacgcgca ctgattgtta ccgatcgttt tctgtttaat aacggctatg 1501 cagaccagat tacctctgtt ctgaaagcgg caggcgttga aaccgaagtt ttttttgaag 1561 ttgaagcaga tccgacactg agcattgttc gtaaaggtgc ggaactggcc aatagcttta 1621 aaccggatgt tattattgcg ctgggtggtg gcagcccgat ggatgcagcc aaaattatgt 1681 gggttatgta tgaacatccc gaaacccatt ttgaagaact ggcgctgcgt tttatggata 1741 ttcgtaaacg catttacaaa tttccgaaga tgggtgtgaa agccaagatg attgccgtta 1801 ccaccacctc aggcaccggt agcgaagtga ccccgtttgc cgttgtgacc gatgatgcca 1861 ccggtcagaa atatcctctg gcagattatg cactgacacc ggatatggcc attgttgatg 1921 caaatctggt gatggatatg ccgaaatcac tgtgtgcatt tggtggcctg gatgcggtta 1981 cccatgcaat ggaagcatat gtgagcgtgc tggcgagcga atttagtgat ggtcaggcac 2041 tgcaagccct gaaactgctg aaagaatatt taccggcatc atatcatgag ggcagcaaaa 2101 atccggttgc acgtgaacgc gtgcatagcg cagcaaccat tgcaggtatt gcatttgcca 2161 atgcatttct gggtgtttgt catagcatgg cacacaaact gggtagtcag tttcatattc 2221 cgcatggtct ggctaatgcg ctgctgattt gtaatgttat tcgctacaat gccaacgaca 2281 acccgaccaa acagaccgcc tttagccagt atgatcgtcc gcaggcacgt cgtcgttatg 2341 cagaaattgc cgatcatctg ggtctgagcg caccgggtga tcgcacagcc gcaaaaattg 2401 aaaaattact ggcatggctg gaaaccctga aagctgaact gggcattccg aaaagcattc 2461 gtgaagccgg tgttcaagaa gcagactttc tggcgaatgt tgataaactg tcggaagatg 2521 catttgatga tcagtgtaca ggtgcaaatc cgcgttatcc gctgattagt gaactgaaac 2581 aaattctgct ggatacctat tatggtcgcg attatgttga aggcgaaacc gcagcgaaaa 2641 aagaagccgc tccggcaaaa gccgaaaaaa aggcaaaaaa atcagcctaa tactagtagc 2701 ggccgctgca g //