##gff-version 3
##sequence-region BBa_J100109 1 2711
BBa_J100109	.	sequence_feature	14	17	.	+	0	ID=annotation2215273;Name=BsaI sticky end
BBa_J100109	.	engineered_region	2692	2711	.	+	0	ID=annotation2215270;Name=suffix
BBa_J100109	.	stop_codon	2688	2690	.	+	0	ID=annotation2215276;Name=stop
BBa_J100109	.	sequence_feature	7	12	.	+	0	ID=annotation2215272;Name=BsaI cuts right
BBa_J100109	.	sequence_feature	1	6	.	+	0	ID=annotation2215271;Name=EcoRI site
BBa_J100109	.	start_codon	15	17	.	+	0	ID=annotation2215274;Name=start
BBa_J100109	.	CDS	15	2687	.	+	0	ID=annotation2215275;Name=adhE allele 
>BBa_J100109
gaattcggtctcgaatggcagttaccaatgttgcagaactgaatgcactggttgaacgtgttaaaaaagcacagcgtga
atatgcaagctttacccaagaacaggtggacaaaatctttcgtgcagcagcactggcagcagccgatgcacgtattccg
ctggcaaaaatggccgttgccgaaagcggtatgggtattgttgaagataaagtgatcaaaaaccacttcgccagcgagt
atatctataacgcctataaagatgagaaaacctgtggtgttctgagcgaagatgatacctttggcaccattaccattgc
cgaaccgattggcattatttgtggtattgtgccgaccaccaatccgaccagcaccgcaatctttaaaagcctgattagc
ctgaaaacccgcaacgcaattatctttagtccgcatccgcgtgcaaaagatgcaaccaataaagcagcagatattgtgc
tgcaagcagcaattgcagcgggtgctccgaaagatctgattggttggattgatcagccgagcgttgaactgagcaatgc
cctgatgcaccatccggatattaatctgattctggcaaccggtggtccgggtatggttaaagccgcatatagcagcggt
aaaccggcaattggtgttggtgcaggtaatactccggttgttattgatgaaaccgctgatattaaacgtgcagttgcaa
gcgttctgatgagcaaaacatttgataatggtgttatttgcgcaagcgaacagagcgttgttgttgtggatagcgttta
tgatgcagttcgtgaacgttttgcaacccatggtggttacctgttacagggtaaagagctgaaagcagttcaggatgtg
attctgaaaaatggtgcgctgaatgccgcaattgttggtcagcctgcgtataaaatcgccgaactggcaggttttagcg
ttccggaaaacaccaaaattctgatcggtgaagttaccgtggtggatgaaagcgaaccgtttgcacatgaaaaactgag
cccgaccctggcaatgtatcgtgcgaaagactttgaagatgccgttgaaaaagcagagaaactggttgcaatgggtggc
attggtcataccagctgtctgtatacggatcaggataatcaaccggcacgtgttagctattttggccagaaaatgaaaa
cagcacgcattctgattaatacaccggcaagccagggtggtatcggtgatctgtataactttaaactggcaccgagtct
gaccctgggttgtggtagctggggtggtaatagcattagcgaaaatgttggtccgaaacacctgatcaacaaaaaaacc
gttgccaaacgtgccgaaaatatgctgtggcataaactgcctaaaagcatctattttcgtcgtggtagcctgccgattg
ccctggatgaagttattaccgatggtcacaaacgcgcactgattgttaccgatcgttttctgtttaataacggctatgc
agaccagattacctctgttctgaaagcggcaggcgttgaaaccgaagttttttttgaagttgaagcagatccgacactg
agcattgttcgtaaaggtgcggaactggccaatagctttaaaccggatgttattattgcgctgggtggtggcagcccga
tggatgcagccaaaattatgtgggttatgtatgaacatcccgaaacccattttgaagaactggcgctgcgttttatgga
tattcgtaaacgcatttacaaatttccgaagatgggtgtgaaagccaagatgattgccgttaccaccacctcaggcacc
ggtagcgaagtgaccccgtttgccgttgtgaccgatgatgccaccggtcagaaatatcctctggcagattatgcactga
caccggatatggccattgttgatgcaaatctggtgatggatatgccgaaatcactgtgtgcatttggtggcctggatgc
ggttacccatgcaatggaagcatatgtgagcgtgctggcgagcgaatttagtgatggtcaggcactgcaagccctgaaa
ctgctgaaagaatatttaccggcatcatatcatgagggcagcaaaaatccggttgcacgtgaacgcgtgcatagcgcag
caaccattgcaggtattgcatttgccaatgcatttctgggtgtttgtcatagcatggcacacaaactgggtagtcagtt
tcatattccgcatggtctggctaatgcgctgctgatttgtaatgttattcgctacaatgccaacgacaacccgaccaaa
cagaccgcctttagccagtatgatcgtccgcaggcacgtcgtcgttatgcagaaattgccgatcatctgggtctgagcg
caccgggtgatcgcacagccgcaaaaattgaaaaattactggcatggctggaaaccctgaaagctgaactgggcattcc
gaaaagcattcgtgaagccggtgttcaagaagcagactttctggcgaatgttgataaactgtcggaagatgcatttgat
gatcagtgtacaggtgcaaatccgcgttatccgctgattagtgaactgaaacaaattctgctggatacctattatggtc
gcgattatgttgaaggcgaaaccgcagcgaaaaaagaagccgctccggcaaaagccgaaaaaaaggcaaaaaaatcagc
ctaatactagtagcggccgctgcag