BBa_J100109 1 BBa_J100109 adhE optimized for E. coli, PstI removed 2013-04-17T11:00:00Z 2015-08-31T04:08:22Z gi|49175990:1294669-1297344 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome Reverse strand. This CDS codes for the adhE gene from E. coli and can be used in conjunction with an adhE- strain for complementation. This has been designed to go downstream of a C-Dog RBS using golden gate assembly (GGA) with BsaI as the restriction enzyme. false false _578_ 0 201 61 Not in stock false Had to remove 4 internal PstI sites. Codons were optimized for E. coli. false Malcolm Campbell annotation2215273 1 BsaI sticky end range2215273 1 14 17 annotation2215274 1 start range2215274 1 15 17 annotation2215270 1 suffix range2215270 1 2692 2711 annotation2215276 1 stop range2215276 1 2688 2690 annotation2215271 1 EcoRI site range2215271 1 1 6 annotation2215275 1 adhE allele range2215275 1 15 2687 annotation2215272 1 BsaI cuts right range2215272 1 7 12 BBa_J100109_sequence 1 gaattcggtctcgaatggcagttaccaatgttgcagaactgaatgcactggttgaacgtgttaaaaaagcacagcgtgaatatgcaagctttacccaagaacaggtggacaaaatctttcgtgcagcagcactggcagcagccgatgcacgtattccgctggcaaaaatggccgttgccgaaagcggtatgggtattgttgaagataaagtgatcaaaaaccacttcgccagcgagtatatctataacgcctataaagatgagaaaacctgtggtgttctgagcgaagatgatacctttggcaccattaccattgccgaaccgattggcattatttgtggtattgtgccgaccaccaatccgaccagcaccgcaatctttaaaagcctgattagcctgaaaacccgcaacgcaattatctttagtccgcatccgcgtgcaaaagatgcaaccaataaagcagcagatattgtgctgcaagcagcaattgcagcgggtgctccgaaagatctgattggttggattgatcagccgagcgttgaactgagcaatgccctgatgcaccatccggatattaatctgattctggcaaccggtggtccgggtatggttaaagccgcatatagcagcggtaaaccggcaattggtgttggtgcaggtaatactccggttgttattgatgaaaccgctgatattaaacgtgcagttgcaagcgttctgatgagcaaaacatttgataatggtgttatttgcgcaagcgaacagagcgttgttgttgtggatagcgtttatgatgcagttcgtgaacgttttgcaacccatggtggttacctgttacagggtaaagagctgaaagcagttcaggatgtgattctgaaaaatggtgcgctgaatgccgcaattgttggtcagcctgcgtataaaatcgccgaactggcaggttttagcgttccggaaaacaccaaaattctgatcggtgaagttaccgtggtggatgaaagcgaaccgtttgcacatgaaaaactgagcccgaccctggcaatgtatcgtgcgaaagactttgaagatgccgttgaaaaagcagagaaactggttgcaatgggtggcattggtcataccagctgtctgtatacggatcaggataatcaaccggcacgtgttagctattttggccagaaaatgaaaacagcacgcattctgattaatacaccggcaagccagggtggtatcggtgatctgtataactttaaactggcaccgagtctgaccctgggttgtggtagctggggtggtaatagcattagcgaaaatgttggtccgaaacacctgatcaacaaaaaaaccgttgccaaacgtgccgaaaatatgctgtggcataaactgcctaaaagcatctattttcgtcgtggtagcctgccgattgccctggatgaagttattaccgatggtcacaaacgcgcactgattgttaccgatcgttttctgtttaataacggctatgcagaccagattacctctgttctgaaagcggcaggcgttgaaaccgaagttttttttgaagttgaagcagatccgacactgagcattgttcgtaaaggtgcggaactggccaatagctttaaaccggatgttattattgcgctgggtggtggcagcccgatggatgcagccaaaattatgtgggttatgtatgaacatcccgaaacccattttgaagaactggcgctgcgttttatggatattcgtaaacgcatttacaaatttccgaagatgggtgtgaaagccaagatgattgccgttaccaccacctcaggcaccggtagcgaagtgaccccgtttgccgttgtgaccgatgatgccaccggtcagaaatatcctctggcagattatgcactgacaccggatatggccattgttgatgcaaatctggtgatggatatgccgaaatcactgtgtgcatttggtggcctggatgcggttacccatgcaatggaagcatatgtgagcgtgctggcgagcgaatttagtgatggtcaggcactgcaagccctgaaactgctgaaagaatatttaccggcatcatatcatgagggcagcaaaaatccggttgcacgtgaacgcgtgcatagcgcagcaaccattgcaggtattgcatttgccaatgcatttctgggtgtttgtcatagcatggcacacaaactgggtagtcagtttcatattccgcatggtctggctaatgcgctgctgatttgtaatgttattcgctacaatgccaacgacaacccgaccaaacagaccgcctttagccagtatgatcgtccgcaggcacgtcgtcgttatgcagaaattgccgatcatctgggtctgagcgcaccgggtgatcgcacagccgcaaaaattgaaaaattactggcatggctggaaaccctgaaagctgaactgggcattccgaaaagcattcgtgaagccggtgttcaagaagcagactttctggcgaatgttgataaactgtcggaagatgcatttgatgatcagtgtacaggtgcaaatccgcgttatccgctgattagtgaactgaaacaaattctgctggatacctattatggtcgcgattatgttgaaggcgaaaccgcagcgaaaaaagaagccgctccggcaaaagccgaaaaaaaggcaaaaaaatcagcctaatactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z