BBa_J100113 1 BBa_J100113 sfGFP cloned into J100112 2013-07-02T11:00:00Z 2015-08-31T04:08:23Z All parts taken from Registry. sfGFP (J100070) ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put the sfGFP into the gap between junctions C and D. iPCR was used to generate the receiving plasmid. false false _578_ 0 18047 9 Not in stock false No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100113. false jessica gronniger annotation2330209 1 sfGFP (J100070) range2330209 1 170 886 annotation2330205 1 P5 (J119031) range2330205 1 18 54 annotation2330207 1 C. dog BD24 (J119028) range2330207 1 73 151 annotation2330208 1 Junction C range2330208 1 152 169 annotation2330210 1 Junction D range2330210 1 887 904 annotation2330204 1 Junction A range2330204 1 1 17 annotation2330206 1 Junction B range2330206 1 55 72 BBa_J100113_sequence 1 atagtctgttcatggtgcttgacaattaatcatccggctcgtaatttatgtggaggctgatgtagttaaggcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacgggttctaatggggaccatccgtaaaggtgaagaactgtttaccggtgttgttccgattctggttgaactggatggtgatgttaatggccacaaattttcagttcgtggtgaaggcgaaggtgatgcaaccaatggtaaactgaccctgaaatttatctgtaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgttttgcacgttatccggatcatatgaaacagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatctcctttaaagatgatggcacctataaaacccgtgccgaagttaaatttgaaggtgacaccctggtgaatcgtattgagctgaaaggcatcgatttcaaagaggatggtaatatcctgggccataaactggaatataacttcaatagccacaatgtgtatatcaccgcagacaaacagaaaaacggcattaaagccaactttaagattcgccataatgtggaagatggtagcgtgcagctggcagatcattatcagcagaataccccgattggtgatggtccggttctgctgccggataatcactatctgagcacccagagcgttctgagcaaagatccgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccatggtatggatgaactgtacaaataatgattaagctatgtattcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z