LOCUS BBa_J100114 4150 bp DNA linear 22-Nov-2024 DEFINITION eCDM8 and GFP Riboswitch into pSB1A8 ACCESSION BBa_J100114 VERSION BBa_J100114.1 FEATURES Location/Qualifiers promoter 1..36 /label=P5 Promoter RBS 37..124 /label=BD2 Bicistronic Translational Junction misc_feature 122..3271 /label=eCDM8 misc_feature 3269..3271 /label=Stop promoter 3272..3322 /label=T5 Promoter misc_feature 3429..4150 /label=Superfolder GFP misc_feature 3373..3430 /label=Riboswitch ORIGIN 1 ttgacaatta atcatccggc tcgtaattta tgtggagggc ccaagttcac ttaaaaagga 61 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gaggttttct 121 aatgaccatc aaagaaatgc cgcagccgaa aacctttggt gaactgaaaa atctgccgct 181 gctgaatacc gataaaccgg ttcatgcact gatgaaaatt gcagatgaac tgggcgagat 241 ctttaaattc gaagcaccgg gtagcgttac ccgttatctg agcagccagc gtctgattaa 301 agaagcatgc gacgaaagcc gctttgataa aaatctgatt cagtggctga aattcatccg 361 cgattttctg ggtgatggtc tgagcaccag ctggacccat gaaaaaaact ggaaaaaagc 421 ccataatatt ctgctgccga gctttagcca gcaggcaatg aaaggttatc atgcaatgat 481 ggttgatatt gccgttcagc tggttcagaa atgggaacgt ctgaatgcag atgaacatat 541 tgaagttccg gaagatatga cccgtctgac cctggatacc attggtctgt gtggttttaa 601 ctatcgcttc aacagctttt atcgtgatca gccgcatccg tttgttacca gcatggttcg 661 tgcactggat gaagcagtga ataaatggca gcgtgcaaat ccggatgatc cggcatatga 721 tgaaaataaa cgtcagttcc aagaggatat taaagtgatg aatgacctgg tggacaaaat 781 tatcgcagat cgtaaagcaa gcggtgaaca gagtgatgat ctgctgaccc atatgctgaa 841 tggtaaagat ccggaaaccg gtgaaccgct ggatgatgaa aacattcgtt atcagattat 901 taccttcctg attgccggtc atgaaaccac cagtggtctg ctgagctttg cactgtattt 961 tctggttaaa aatccgcacg ttctgcaaaa agcagccgaa gaagcagcac gcgttctggt 1021 tgatccggtt ccgagctata aacaggttaa acagctgaaa tatgtgggca tggttctgaa 1081 tgaagcactg cgtctgtggc cgacctttcc gtggtttagc ctgtatgcaa aagaagatac 1141 cgttctggga ggtgaatatc cgctggaaaa aggtgatgaa ctgatggttc tgattccgca 1201 gctgcatcgt gataaaacca tttggggtga tgatgtggaa gaatttcgtc cggaacgttt 1261 tgaaaatccg agcgcaattc cgcagcatgc ctttaaaccg tttggtaatg gtcagcgtgc 1321 atgtattggt cagcagtttg cactgcatga agccaccctg gttctgggta tgatgctgaa 1381 acattttgat tttgaggacc acaccaacta tgagctggat attaaaggca ccctgaccct 1441 gaaaccggaa ggttttgttg ttaaagccaa aagcaaaaaa atcccgctgg gtggtattcc 1501 gagcccgagc accgaacaga gcgcaaaaaa agttcgtaaa aaagccgaaa acgcccataa 1561 tacaccgctg ctggttctgt atggtagcaa tatgggtaca gccgaaggca ccgcacgtga 1621 tctggcagat attgcaatga gcaaaggttt tgcaccgcag gttgcaaccc tggatagcca 1681 tgcaggtaat ctgcctcgtg aaggtgcagt tctgattgtt accgcaagct ataatggtca 1741 tccgcctgat aatgcaaaac agtttgttga ttggctggat caggcaagcg cagatgaagt 1801 taaaggtgtt cgttatagcg tttttggttg cggtgataaa aactgggcaa ccacctatca 1861 gaaagttccg gcatttattg atgaaaccct ggcagcaaaa ggtgcagaaa atattgcaga 1921 tcgtggtgaa accgatgcca gtgatgattt tgaaggcacc tatgaagaat ggcgtgaaca 1981 tatgtggtca gatgttgcag cctattttaa cctggatatc gaaaacagcg aggacaataa 2041 aagcaccctg agcctgcaat ttgtggatag cgcagcagat atgccgctgg caaaaatgca 2101 tggtgcattt agcaccaatg ttgttgcaag caaagaactg caacagcctg gtagcgcacg 2161 tagcacccgt catctggaaa ttgaactgcc gaaagaagca agctatcaag agggtgatca 2221 tctgggtgtt attccgcgta attatgaagg tattgttaat cgtgttaccg cacgttttgg 2281 tctggatgca agccagcaga ttcgtctgga agcagaagaa gaaaaactgg cacatctgcc 2341 gctggccaaa accgttagcg ttgaagaact gctgcaatat gttgaactgc aagatccggt 2401 tacccgtacc cagctgcgtg caatggcagc aaaaaccgtt tgtccgcctc ataaagttga 2461 actggaagca ctgctggaga aacaggcata taaagaacag gttctggcaa aacgtctgac 2521 catgctggaa ctgctggaaa aatatccggc atgcgaaatg aaattcagcg aatttattgc 2581 actgctgccg agtattcgtc cgcgttatta tagcattagc agcagtccgc gtgttgatga 2641 aaaacaggcc agcattaccg ttagcgtggt tagcggtgaa gcatggtcag gttatggtga 2701 atataaaggt attgccagca attatctggc cgaactgcaa gaaggtgata ccattacctg 2761 ttttattagc acaccgcaga gcgaatttac cctgccgaaa gatcctgaaa caccgctgat 2821 tatggttggt ccgggtacag gtgttgcacc gtttcgtggt tttgttcagg cacgtaaaca 2881 actgaaagaa cagggtcaga gcctgggtga agcacatctg tattttggtt gtcgtagtcc 2941 gcatgaggat tatctgtatc aagaagaact ggaaaacgca cagagcgaag gtattattac 3001 cctgcatacc gcatttagcc gtatgccgaa tcagccgaaa acatatgttc agcatgttat 3061 ggaacaggat ggcaaaaaac tgattgaact gctggatcag ggtgcccatt tctatatttg 3121 tggtgatggt agccagatgg caccggcagt tgaagcaacc ctgatgaaaa gctatgcaga 3181 tgttcatcag gttagcgaag cagatgcacg tctgtggctg caacagctgg aagaaaaagg 3241 tcgttatgca aaagatgttt gggcaggtta aaaatcataa aaaatttatt tgctttgtga 3301 gcggataaca attataatag attcaattgt gagcggataa caattactag agatacgact 3361 cactataggt accggtgata ccagcatcgt cttgatgccc ttggcagcac cctgctaagg 3421 taacaacaag atgcgtaaag gcgaagagct gttcaccggt gttgttccga ttctggttga 3481 actggatggt gatgttaatg gccacaaatt ttcagttcgt ggtgaaggcg agggtgatgc 3541 aaccaatggt aaactgaccc tgaaatttat ctgtaccacc ggcaaactgc cggttccgtg 3601 gccgaccctg gttaccaccc tgacctatgg tgttcagtgt tttgcacgtt atccggatca 3661 tatgaaacag cacgattttt tcaaaagcgc aatgccggaa ggttatgttc aagaacgtac 3721 catctccttt aaagatgatg gcacctataa aacccgtgcc gaagttaaat ttgaaggtga 3781 caccctggtg aatcgtattg agctgaaagg catcgatttc aaagaggatg gtaatatcct 3841 gggccataaa ctggaatata acttcaatag ccacaatgtg tatatcaccg cagacaaaca 3901 gaaaaacggc attaaagcca actttaagat tcgccataat gtggaagatg gtagcgtgca 3961 gctggcagat cattatcagc agaatacccc gattggtgat ggtccggttc tgctgccgga 4021 taatcactat ctgagcaccc agagcgttct gagcaaagat ccgaatgaaa aacgtgatca 4081 catggtgctg ctggaatttg ttaccgcagc aggtattacc catggtatgg atgaactgta 4141 caaatgatga //