LOCUS       BBa_J100116             2881 bp    DNA     linear       22-Nov-2024
DEFINITION  AdhE gene cloned into J100112
ACCESSION   BBa_J100116
VERSION     BBa_J100116.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=Junction A
     promoter        18..54
                     /label=P5 (J119031)
     misc_feature    55..72
                     /label=Junction B
     RBS             73..151
                     /label=C. dog BD24 (J119028)
     misc_feature    152..169
                     /label=Junction C
     misc_feature    170..2862
                     /label=adhE (J100109) 
     misc_feature    2863..2881
                     /label=Junction D
ORIGIN
        1 atagtctgtt catggtgctt gacaattaat catccggctc gtaatttatg tggaggctga
       61 tgtagttaag gcgggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg
      121 tactgaaaca tcttaatcat gcgatggacg ggttctaatg gggaccatcg cagttaccaa
      181 tgttgcagaa ctgaatgcac tggttgaacg tgttaaaaaa gcacagcgtg aatatgcaag
      241 ctttacccaa gaacaggtgg acaaaatctt tcgtgcagca gcactggcag cagccgatgc
      301 acgtattccg ctggcaaaaa tggccgttgc cgaaagcggt atgggtattg ttgaagataa
      361 agtgatcaaa aaccacttcg ccagcgagta tatctataac gcctataaag atgagaaaac
      421 ctgtggtgtt ctgagcgaag atgatacctt tggcaccatt accattgccg aaccgattgg
      481 cattatttgt ggtattgtgc cgaccaccaa tccgaccagc accgcaatct ttaaaagcct
      541 gattagcctg aaaacccgca acgcaattat ctttagtccg catccgcgtg caaaagatgc
      601 aaccaataaa gcagcagata ttgtgctgca agcagcaatt gcagcgggtg ctccgaaaga
      661 tctgattggt tggattgatc agccgagcgt tgaactgagc aatgccctga tgcaccatcc
      721 ggatattaat ctgattctgg caaccggtgg tccgggtatg gttaaagccg catatagcag
      781 cggtaaaccg gcaattggtg ttggtgcagg taatactccg gttgttattg atgaaaccgc
      841 tgatattaaa cgtgcagttg caagcgttct gatgagcaaa acatttgata atggtgttat
      901 ttgcgcaagc gaacagagcg ttgttgttgt ggatagcgtt tatgatgcag ttcgtgaacg
      961 ttttgcaacc catggtggtt acctgttaca gggtaaagag ctgaaagcag ttcaggatgt
     1021 gattctgaaa aatggtgcgc tgaatgccgc aattgttggt cagcctgcgt ataaaatcgc
     1081 cgaactggca ggttttagcg ttccggaaaa caccaaaatt ctgatcggtg aagttaccgt
     1141 ggtggatgaa agcgaaccgt ttgcacatga aaaactgagc ccgaccctgg caatgtatcg
     1201 tgcgaaagac tttgaagatg ccgttgaaaa agcagagaaa ctggttgcaa tgggtggcat
     1261 tggtcatacc agctgtctgt atacggatca ggataatcaa ccggcacgtg ttagctattt
     1321 tggccagaaa atgaaaacag cacgcattct gattaataca ccggcaagcc agggtggtat
     1381 cggtgatctg tataacttta aactggcacc gagtctgacc ctgggttgtg gtagctgggg
     1441 tggtaatagc attagcgaaa atgttggtcc gaaacacctg atcaacaaaa aaaccgttgc
     1501 caaacgtgcc gaaaatatgc tgtggcataa actgcctaaa agcatctatt ttcgtcgtgg
     1561 tagcctgccg attgccctgg atgaagttat taccgatggt cacaaacgcg cactgattgt
     1621 taccgatcgt tttctgttta ataacggcta tgcagaccag attacctctg ttctgaaagc
     1681 ggcaggcgtt gaaaccgaag ttttttttga agttgaagca gatccgacac tgagcattgt
     1741 tcgtaaaggt gcggaactgg ccaatagctt taaaccggat gttattattg cgctgggtgg
     1801 tggcagcccg atggatgcag ccaaaattat gtgggttatg tatgaacatc ccgaaaccca
     1861 ttttgaagaa ctggcgctgc gttttatgga tattcgtaaa cgcatttaca aatttccgaa
     1921 gatgggtgtg aaagccaaga tgattgccgt taccaccacc tcaggcaccg gtagcgaagt
     1981 gaccccgttt gccgttgtga ccgatgatgc caccggtcag aaatatcctc tggcagatta
     2041 tgcactgaca ccggatatgg ccattgttga tgcaaatctg gtgatggata tgccgaaatc
     2101 actgtgtgca tttggtggcc tggatgcggt tacccatgca atggaagcat atgtgagcgt
     2161 gctggcgagc gaatttagtg atggtcaggc actgcaagcc ctgaaactgc tgaaagaata
     2221 tttaccggca tcatatcatg agggcagcaa aaatccggtt gcacgtgaac gcgtgcatag
     2281 cgcagcaacc attgcaggta ttgcatttgc caatgcattt ctgggtgttt gtcatagcat
     2341 ggcacacaaa ctgggtagtc agtttcatat tccgcatggt ctggctaatg cgctgctgat
     2401 ttgtaatgtt attcgctaca atgccaacga caacccgacc aaacagaccg cctttagcca
     2461 gtatgatcgt ccgcaggcac gtcgtcgtta tgcagaaatt gccgatcatc tgggtctgag
     2521 cgcaccgggt gatcgcacag ccgcaaaaat tgaaaaatta ctggcatggc tggaaaccct
     2581 gaaagctgaa ctgggcattc cgaaaagcat tcgtgaagcc ggtgttcaag aagcagactt
     2641 tctggcgaat gttgataaac tgtcggaaga tgcatttgat gatcagtgta caggtgcaaa
     2701 tccgcgttat ccgctgatta gtgaactgaa acaaattctg ctggatacct attatggtcg
     2761 cgattatgtt gaaggcgaaa ccgcagcgaa aaaagaagcc gctccggcaa aagccgaaaa
     2821 aaaggcaaaa aaatcagcct aatactagta gcggccgctg cagcggagct atgtattcct
     2881 g
//