BBa_J100116 1 BBa_J100116 AdhE gene cloned into J100112 2013-07-16T11:00:00Z 2015-08-31T04:08:23Z All parts taken from Registry. AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put adhE into the gap between junctions C and D. iPCR was used to generate the receiving plasmid. false false _578_ 0 18047 9 Not in stock false No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100116. false jessica gronniger annotation2330922 1 Junction D range2330922 1 2863 2881 annotation2330920 1 Junction C range2330920 1 152 169 annotation2330919 1 C. dog BD24 (J119028) range2330919 1 73 151 annotation2330918 1 Junction B range2330918 1 55 72 annotation2330921 1 adhE (J100109) range2330921 1 170 2862 annotation2330916 1 Junction A range2330916 1 1 17 annotation2330917 1 P5 (J119031) range2330917 1 18 54 BBa_J100116_sequence 1 atagtctgttcatggtgcttgacaattaatcatccggctcgtaatttatgtggaggctgatgtagttaaggcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacgggttctaatggggaccatcgcagttaccaatgttgcagaactgaatgcactggttgaacgtgttaaaaaagcacagcgtgaatatgcaagctttacccaagaacaggtggacaaaatctttcgtgcagcagcactggcagcagccgatgcacgtattccgctggcaaaaatggccgttgccgaaagcggtatgggtattgttgaagataaagtgatcaaaaaccacttcgccagcgagtatatctataacgcctataaagatgagaaaacctgtggtgttctgagcgaagatgatacctttggcaccattaccattgccgaaccgattggcattatttgtggtattgtgccgaccaccaatccgaccagcaccgcaatctttaaaagcctgattagcctgaaaacccgcaacgcaattatctttagtccgcatccgcgtgcaaaagatgcaaccaataaagcagcagatattgtgctgcaagcagcaattgcagcgggtgctccgaaagatctgattggttggattgatcagccgagcgttgaactgagcaatgccctgatgcaccatccggatattaatctgattctggcaaccggtggtccgggtatggttaaagccgcatatagcagcggtaaaccggcaattggtgttggtgcaggtaatactccggttgttattgatgaaaccgctgatattaaacgtgcagttgcaagcgttctgatgagcaaaacatttgataatggtgttatttgcgcaagcgaacagagcgttgttgttgtggatagcgtttatgatgcagttcgtgaacgttttgcaacccatggtggttacctgttacagggtaaagagctgaaagcagttcaggatgtgattctgaaaaatggtgcgctgaatgccgcaattgttggtcagcctgcgtataaaatcgccgaactggcaggttttagcgttccggaaaacaccaaaattctgatcggtgaagttaccgtggtggatgaaagcgaaccgtttgcacatgaaaaactgagcccgaccctggcaatgtatcgtgcgaaagactttgaagatgccgttgaaaaagcagagaaactggttgcaatgggtggcattggtcataccagctgtctgtatacggatcaggataatcaaccggcacgtgttagctattttggccagaaaatgaaaacagcacgcattctgattaatacaccggcaagccagggtggtatcggtgatctgtataactttaaactggcaccgagtctgaccctgggttgtggtagctggggtggtaatagcattagcgaaaatgttggtccgaaacacctgatcaacaaaaaaaccgttgccaaacgtgccgaaaatatgctgtggcataaactgcctaaaagcatctattttcgtcgtggtagcctgccgattgccctggatgaagttattaccgatggtcacaaacgcgcactgattgttaccgatcgttttctgtttaataacggctatgcagaccagattacctctgttctgaaagcggcaggcgttgaaaccgaagttttttttgaagttgaagcagatccgacactgagcattgttcgtaaaggtgcggaactggccaatagctttaaaccggatgttattattgcgctgggtggtggcagcccgatggatgcagccaaaattatgtgggttatgtatgaacatcccgaaacccattttgaagaactggcgctgcgttttatggatattcgtaaacgcatttacaaatttccgaagatgggtgtgaaagccaagatgattgccgttaccaccacctcaggcaccggtagcgaagtgaccccgtttgccgttgtgaccgatgatgccaccggtcagaaatatcctctggcagattatgcactgacaccggatatggccattgttgatgcaaatctggtgatggatatgccgaaatcactgtgtgcatttggtggcctggatgcggttacccatgcaatggaagcatatgtgagcgtgctggcgagcgaatttagtgatggtcaggcactgcaagccctgaaactgctgaaagaatatttaccggcatcatatcatgagggcagcaaaaatccggttgcacgtgaacgcgtgcatagcgcagcaaccattgcaggtattgcatttgccaatgcatttctgggtgtttgtcatagcatggcacacaaactgggtagtcagtttcatattccgcatggtctggctaatgcgctgctgatttgtaatgttattcgctacaatgccaacgacaacccgaccaaacagaccgcctttagccagtatgatcgtccgcaggcacgtcgtcgttatgcagaaattgccgatcatctgggtctgagcgcaccgggtgatcgcacagccgcaaaaattgaaaaattactggcatggctggaaaccctgaaagctgaactgggcattccgaaaagcattcgtgaagccggtgttcaagaagcagactttctggcgaatgttgataaactgtcggaagatgcatttgatgatcagtgtacaggtgcaaatccgcgttatccgctgattagtgaactgaaacaaattctgctggatacctattatggtcgcgattatgttgaaggcgaaaccgcagcgaaaaaagaagccgctccggcaaaagccgaaaaaaaggcaaaaaaatcagcctaatactagtagcggccgctgcagcggagctatgtattcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z