BBa_J100120 1 BBa_J100120 J100116 with P5 Promoter Deleted 2013-07-28T11:00:00Z 2015-08-31T04:08:23Z All parts taken from Registry. AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). C. dog BD24 (J119028) was ligated between junctions B and C on scaffold. P5 promoter between junctions A and B was deleted. Golden Gate assembly was used to put the C. dog and adhE into the scaffold. iPCR was used to generate the receiving plasmid. false false _578_ 0 18047 9 Not in stock false No internal BsaI sites. false jessica gronniger annotation2331189 1 Junction D range2331189 1 2827 2845 annotation2331187 1 Junction C range2331187 1 116 133 annotation2331186 1 C. dog (J119028) range2331186 1 36 115 annotation2331184 1 Junction A range2331184 1 1 17 annotation2331188 1 adhE (J100109) range2331188 1 134 2826 annotation2331185 1 Junction B range2331185 1 18 35 BBa_J100120_sequence 1 atagtctgttcatggtgcggctgatgtagttaaggcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacgggttctaatggggaccatcgcagttaccaatgttgcagaactgaatgcactggttgaacgtgttaaaaaagcacagcgtgaatatgcaagctttacccaagaacaggtggacaaaatctttcgtgcagcagcactggcagcagccgatgcacgtattccgctggcaaaaatggccgttgccgaaagcggtatgggtattgttgaagataaagtgatcaaaaaccacttcgccagcgagtatatctataacgcctataaagatgagaaaacctgtggtgttctgagcgaagatgatacctttggcaccattaccattgccgaaccgattggcattatttgtggtattgtgccgaccaccaatccgaccagcaccgcaatctttaaaagcctgattagcctgaaaacccgcaacgcaattatctttagtccgcatccgcgtgcaaaagatgcaaccaataaagcagcagatattgtgctgcaagcagcaattgcagcgggtgctccgaaagatctgattggttggattgatcagccgagcgttgaactgagcaatgccctgatgcaccatccggatattaatctgattctggcaaccggtggtccgggtatggttaaagccgcatatagcagcggtaaaccggcaattggtgttggtgcaggtaatactccggttgttattgatgaaaccgctgatattaaacgtgcagttgcaagcgttctgatgagcaaaacatttgataatggtgttatttgcgcaagcgaacagagcgttgttgttgtggatagcgtttatgatgcagttcgtgaacgttttgcaacccatggtggttacctgttacagggtaaagagctgaaagcagttcaggatgtgattctgaaaaatggtgcgctgaatgccgcaattgttggtcagcctgcgtataaaatcgccgaactggcaggttttagcgttccggaaaacaccaaaattctgatcggtgaagttaccgtggtggatgaaagcgaaccgtttgcacatgaaaaactgagcccgaccctggcaatgtatcgtgcgaaagactttgaagatgccgttgaaaaagcagagaaactggttgcaatgggtggcattggtcataccagctgtctgtatacggatcaggataatcaaccggcacgtgttagctattttggccagaaaatgaaaacagcacgcattctgattaatacaccggcaagccagggtggtatcggtgatctgtataactttaaactggcaccgagtctgaccctgggttgtggtagctggggtggtaatagcattagcgaaaatgttggtccgaaacacctgatcaacaaaaaaaccgttgccaaacgtgccgaaaatatgctgtggcataaactgcctaaaagcatctattttcgtcgtggtagcctgccgattgccctggatgaagttattaccgatggtcacaaacgcgcactgattgttaccgatcgttttctgtttaataacggctatgcagaccagattacctctgttctgaaagcggcaggcgttgaaaccgaagttttttttgaagttgaagcagatccgacactgagcattgttcgtaaaggtgcggaactggccaatagctttaaaccggatgttattattgcgctgggtggtggcagcccgatggatgcagccaaaattatgtgggttatgtatgaacatcccgaaacccattttgaagaactggcgctgcgttttatggatattcgtaaacgcatttacaaatttccgaagatgggtgtgaaagccaagatgattgccgttaccaccacctcaggcaccggtagcgaagtgaccccgtttgccgttgtgaccgatgatgccaccggtcagaaatatcctctggcagattatgcactgacaccggatatggccattgttgatgcaaatctggtgatggatatgccgaaatcactgtgtgcatttggtggcctggatgcggttacccatgcaatggaagcatatgtgagcgtgctggcgagcgaatttagtgatggtcaggcactgcaagccctgaaactgctgaaagaatatttaccggcatcatatcatgagggcagcaaaaatccggttgcacgtgaacgcgtgcatagcgcagcaaccattgcaggtattgcatttgccaatgcatttctgggtgtttgtcatagcatggcacacaaactgggtagtcagtttcatattccgcatggtctggctaatgcgctgctgatttgtaatgttattcgctacaatgccaacgacaacccgaccaaacagaccgcctttagccagtatgatcgtccgcaggcacgtcgtcgttatgcagaaattgccgatcatctgggtctgagcgcaccgggtgatcgcacagccgcaaaaattgaaaaattactggcatggctggaaaccctgaaagctgaactgggcattccgaaaagcattcgtgaagccggtgttcaagaagcagactttctggcgaatgttgataaactgtcggaagatgcatttgatgatcagtgtacaggtgcaaatccgcgttatccgctgattagtgaactgaaacaaattctgctggatacctattatggtcgcgattatgttgaaggcgaaaccgcagcgaaaaaagaagccgctccggcaaaagccgaaaaaaaggcaaaaaaatcagcctaatactagtagcggccgctgcagcggagctatgtattcctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z