LOCUS       BBa_J100122             2833 bp    DNA     linear       26-Jun-2024
DEFINITION  P5+riboswitch+adhE
ACCESSION   BBa_J100122
VERSION     BBa_J100122.1  
FEATURES             Location/Qualifiers
     promoter        1..53
                     /label=P5 Promoter
     RBS             54..75
                     /label=lac operator
     RBS             76..81
                     /label=scar
     RBS             83..97
                     /label=constant sequence
     RBS             98..103
                     /label=Knpl site
     RBS             104..163
                     /label=Riboswitch
     RBS             147..150
                     /label=RBS
     CDS             161..2830
                     /label=adhE allele
     misc_feature    176..177
                     /label=deletion one codon (V)
     misc_feature    2831..2833
                     /label=Stop
ORIGIN
        1 gaaatcataa aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt
       61 gagcggataa caattactag agatacgact cactataggt accggtgata ccagcatcgt
      121 cttgatgccc ttggcagcac cctgctaagg taacaacaag atggcagtta ccaatgcaga
      181 actgaatgca ctggttgaac gtgttaaaaa agcacagcgt gaatatgcaa gctttaccca
      241 agaacaggtg gacaaaatct ttcgtgcagc agcactggca gcagccgatg cacgtattcc
      301 gctggcaaaa atggccgttg ccgaaagcgg tatgggtatt gttgaagata aagtgatcaa
      361 aaaccacttc gccagcgagt atatctataa cgcctataaa gatgagaaaa cctgtggtgt
      421 tctgagcgaa gatgatacct ttggcaccat taccattgcc gaaccgattg gcattatttg
      481 tggtattgtg ccgaccacca atccgaccag caccgcaatc tttaaaagcc tgattagcct
      541 gaaaacccgc aacgcaatta tctttagtcc gcatccgcgt gcaaaagatg caaccaataa
      601 agcagcagat attgtgctgc aagcagcaat tgcagcgggt gctccgaaag atctgattgg
      661 ttggattgat cagccgagcg ttgaactgag caatgccctg atgcaccatc cggatattaa
      721 tctgattctg gcaaccggtg gtccgggtat ggttaaagcc gcatatagca gcggtaaacc
      781 ggcaattggt gttggtgcag gtaatactcc ggttgttatt gatgaaaccg ctgatattaa
      841 acgtgcagtt gcaagcgttc tgatgagcaa aacatttgat aatggtgtta tttgcgcaag
      901 cgaacagagc gttgttgttg tggatagcgt ttatgatgca gttcgtgaac gttttgcaac
      961 ccatggtggt tacctgttac agggtaaaga gctgaaagca gttcaggatg tgattctgaa
     1021 aaatggtgcg ctgaatgccg caattgttgg tcagcctgcg tataaaatcg ccgaactggc
     1081 aggttttagc gttccggaaa acaccaaaat tctgatcggt gaagttaccg tggtggatga
     1141 aagcgaaccg tttgcacatg aaaaactgag cccgaccctg gcaatgtatc gtgcgaaaga
     1201 ctttgaagat gccgttgaaa aagcagagaa actggttgca atgggtggca ttggtcatac
     1261 cagctgtctg tatacggatc aggataatca accggcacgt gttagctatt ttggccagaa
     1321 aatgaaaaca gcacgcattc tgattaatac accggcaagc cagggtggta tcggtgatct
     1381 gtataacttt aaactggcac cgagtctgac cctgggttgt ggtagctggg gtggtaatag
     1441 cattagcgaa aatgttggtc cgaaacacct gatcaacaaa aaaaccgttg ccaaacgtgc
     1501 cgaaaatatg ctgtggcata aactgcctaa aagcatctat tttcgtcgtg gtagcctgcc
     1561 gattgccctg gatgaagtta ttaccgatgg tcacaaacgc gcactgattg ttaccgatcg
     1621 ttttctgttt aataacggct atgcagacca gattacctct gttctgaaag cggcaggcgt
     1681 tgaaaccgaa gttttttttg aagttgaagc agatccgaca ctgagcattg ttcgtaaagg
     1741 tgcggaactg gccaatagct ttaaaccgga tgttattatt gcgctgggtg gtggcagccc
     1801 gatggatgca gccaaaatta tgtgggttat gtatgaacat cccgaaaccc attttgaaga
     1861 actggcgctg cgttttatgg atattcgtaa acgcatttac aaatttccga agatgggtgt
     1921 gaaagccaag atgattgccg ttaccaccac ctcaggcacc ggtagcgaag tgaccccgtt
     1981 tgccgttgtg accgatgatg ccaccggtca gaaatatcct ctggcagatt atgcactgac
     2041 accggatatg gccattgttg atgcaaatct ggtgatggat atgccgaaat cactgtgtgc
     2101 atttggtggc ctggatgcgg ttacccatgc aatggaagca tatgtgagcg tgctggcgag
     2161 cgaatttagt gatggtcagg cactgcaagc cctgaaactg ctgaaagaat atttaccggc
     2221 atcatatcat gagggcagca aaaatccggt tgcacgtgaa cgcgtgcata gcgcagcaac
     2281 cattgcaggt attgcatttg ccaatgcatt tctgggtgtt tgtcatagca tggcacacaa
     2341 actgggtagt cagtttcata ttccgcatgg tctggctaat gcgctgctga tttgtaatgt
     2401 tattcgctac aatgccaacg acaacccgac caaacagacc gcctttagcc agtatgatcg
     2461 tccgcaggca cgtcgtcgtt atgcagaaat tgccgatcat ctgggtctga gcgcaccggg
     2521 tgatcgcaca gccgcaaaaa ttgaaaaatt actggcatgg ctggaaaccc tgaaagctga
     2581 actgggcatt ccgaaaagca ttcgtgaagc cggtgttcaa gaagcagact ttctggcgaa
     2641 tgttgataaa ctgtcggaag atgcatttga tgatcagtgt acaggtgcaa atccgcgtta
     2701 tccgctgatt agtgaactga aacaaattct gctggatacc tattatggtc gcgattatgt
     2761 tgaaggcgaa accgcagcga aaaaagaagc cgctccggca aaagccgaaa aaaaggcaaa
     2821 aaaatcagcc taa
//