BBa_J100122 1 BBa_J100122 P5+riboswitch+adhE 2013-09-05T11:00:00Z 2015-08-31T04:08:23Z built from stock parts in Registry. This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31. false false _578_ 0 201 61 Not in stock false Built by Golden Gate Assembly. false Malcolm Campbell annotation2367586 1 Riboswitch range2367586 1 104 163 annotation2367589 1 deletion one codon (V) range2367589 1 176 177 annotation2367588 1 adhE allele range2367588 1 161 2830 annotation2367582 1 lac operator range2367582 1 54 75 annotation2367583 1 scar range2367583 1 76 81 annotation2367590 1 Stop range2367590 1 2831 2833 annotation2367585 1 Knpl site range2367585 1 98 103 annotation2367587 1 RBS range2367587 1 147 150 annotation2367584 1 constant sequence range2367584 1 83 97 annotation2367581 1 P5 Promoter range2367581 1 1 53 BBa_J100122_sequence 1 gaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactagagatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatggcagttaccaatgcagaactgaatgcactggttgaacgtgttaaaaaagcacagcgtgaatatgcaagctttacccaagaacaggtggacaaaatctttcgtgcagcagcactggcagcagccgatgcacgtattccgctggcaaaaatggccgttgccgaaagcggtatgggtattgttgaagataaagtgatcaaaaaccacttcgccagcgagtatatctataacgcctataaagatgagaaaacctgtggtgttctgagcgaagatgatacctttggcaccattaccattgccgaaccgattggcattatttgtggtattgtgccgaccaccaatccgaccagcaccgcaatctttaaaagcctgattagcctgaaaacccgcaacgcaattatctttagtccgcatccgcgtgcaaaagatgcaaccaataaagcagcagatattgtgctgcaagcagcaattgcagcgggtgctccgaaagatctgattggttggattgatcagccgagcgttgaactgagcaatgccctgatgcaccatccggatattaatctgattctggcaaccggtggtccgggtatggttaaagccgcatatagcagcggtaaaccggcaattggtgttggtgcaggtaatactccggttgttattgatgaaaccgctgatattaaacgtgcagttgcaagcgttctgatgagcaaaacatttgataatggtgttatttgcgcaagcgaacagagcgttgttgttgtggatagcgtttatgatgcagttcgtgaacgttttgcaacccatggtggttacctgttacagggtaaagagctgaaagcagttcaggatgtgattctgaaaaatggtgcgctgaatgccgcaattgttggtcagcctgcgtataaaatcgccgaactggcaggttttagcgttccggaaaacaccaaaattctgatcggtgaagttaccgtggtggatgaaagcgaaccgtttgcacatgaaaaactgagcccgaccctggcaatgtatcgtgcgaaagactttgaagatgccgttgaaaaagcagagaaactggttgcaatgggtggcattggtcataccagctgtctgtatacggatcaggataatcaaccggcacgtgttagctattttggccagaaaatgaaaacagcacgcattctgattaatacaccggcaagccagggtggtatcggtgatctgtataactttaaactggcaccgagtctgaccctgggttgtggtagctggggtggtaatagcattagcgaaaatgttggtccgaaacacctgatcaacaaaaaaaccgttgccaaacgtgccgaaaatatgctgtggcataaactgcctaaaagcatctattttcgtcgtggtagcctgccgattgccctggatgaagttattaccgatggtcacaaacgcgcactgattgttaccgatcgttttctgtttaataacggctatgcagaccagattacctctgttctgaaagcggcaggcgttgaaaccgaagttttttttgaagttgaagcagatccgacactgagcattgttcgtaaaggtgcggaactggccaatagctttaaaccggatgttattattgcgctgggtggtggcagcccgatggatgcagccaaaattatgtgggttatgtatgaacatcccgaaacccattttgaagaactggcgctgcgttttatggatattcgtaaacgcatttacaaatttccgaagatgggtgtgaaagccaagatgattgccgttaccaccacctcaggcaccggtagcgaagtgaccccgtttgccgttgtgaccgatgatgccaccggtcagaaatatcctctggcagattatgcactgacaccggatatggccattgttgatgcaaatctggtgatggatatgccgaaatcactgtgtgcatttggtggcctggatgcggttacccatgcaatggaagcatatgtgagcgtgctggcgagcgaatttagtgatggtcaggcactgcaagccctgaaactgctgaaagaatatttaccggcatcatatcatgagggcagcaaaaatccggttgcacgtgaacgcgtgcatagcgcagcaaccattgcaggtattgcatttgccaatgcatttctgggtgtttgtcatagcatggcacacaaactgggtagtcagtttcatattccgcatggtctggctaatgcgctgctgatttgtaatgttattcgctacaatgccaacgacaacccgaccaaacagaccgcctttagccagtatgatcgtccgcaggcacgtcgtcgttatgcagaaattgccgatcatctgggtctgagcgcaccgggtgatcgcacagccgcaaaaattgaaaaattactggcatggctggaaaccctgaaagctgaactgggcattccgaaaagcattcgtgaagccggtgttcaagaagcagactttctggcgaatgttgataaactgtcggaagatgcatttgatgatcagtgtacaggtgcaaatccgcgttatccgctgattagtgaactgaaacaaattctgctggatacctattatggtcgcgattatgttgaaggcgaaaccgcagcgaaaaaagaagccgctccggcaaaagccgaaaaaaaggcaaaaaaatcagcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z