BBa_J100131
1
BBa_J100131
human IL-6
2013-09-22T11:00:00Z
2015-08-31T04:08:23Z
came from GenBank and then was modified.
Human IL-6 CDS optimized for E. coli with no internal restriction sites for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.
false
false
_578_
0
201
61
Not in stock
false
Internal restriction sites removed for EcoRI, XbaI, SpeI, PstI or BsaI. This insert was synthesized by GeneArt and was cloned into their pMK-T plasmid so we had the BioBrick prefix and suffix included in the basic part sequence. It will be used with Golden Gate Assembly to put downstream of promoter+RBS.
false
Malcolm Campbell
annotation2359460
1
sticky end
range2359460
1
592
595
annotation2359024
1
stop codon
range2359024
1
589
591
annotation2359021
1
Prefix
range2359021
1
1
22
annotation2359457
1
BsaI cuts right
range2359457
1
23
28
annotation2359023
1
start codon
range2359023
1
34
36
annotation2359025
1
Human IL-6
range2359025
1
34
591
annotation2359459
1
BsaI cuts left
range2359459
1
597
602
annotation2359458
1
sticky end
range2359458
1
30
33
annotation2359022
1
Suffix
range2359022
1
603
623
BBa_J100131_sequence
1
gaattcgcggccgcttctagagggtctcagcgaatgccggttccgcctggtgaagatagcaaagatgttgcagcatttcatcgtcagccgctgaccagcagcgaacgtattgataaacaaattcgctatatcctggatggcattagcgcactgcgtaaagaaacctgtaataaaagcaatatgtgcgaaagcagcaaagaagcactggcagaaaataatctgaatctgccgaaaatggccgaaaaagatggttgttttcagagcggctttaatgaagaaacctgcctggttaaaatcattaccggtctgctggaatttgaggtgtatctggaatatctgcaaaaccgttttgaaagcagcgaagaacaggcacgtgcagttcagatgagcaccaaagttctgattcagtttctgcaaaaaaaagccaaaaacctggatgcaattaccacaccggatccgaccaccaatgcaagcctgctgaccaaactgcaagcacagaatcagtggctgcaagatatgaccacccatctgattctgcgtagctttaaagaatttctgcaaagcagcctgcgtgcactgcgtcagatgtaagcctagagacctactagtagcggccgctgcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z