LOCUS       BBa_J100132              860 bp    DNA     linear       28-Jun-2024
DEFINITION  E. coli thyA
ACCESSION   BBa_J100132
VERSION     BBa_J100132.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=Prefix
     misc_feature    840..860
                     /label=Suffix
     misc_feature    23..28
                     /label=BsaI cuts right
     misc_feature    30..33
                     /label=sticky end
     misc_feature    834..839
                     /label=BsaI cuts left
     misc_feature    829..832
                     /label=sticky end
     misc_feature    34..36
                     /label=start codon
     misc_feature    826..828
                     /label=stop codon
     CDS             35..828
                     /label=tyA gene
ORIGIN
        1 gaattcgcgg ccgcttctag agggtctcag cgaatgaaac agtatttaga actgatgcaa
       61 aaagtgctcg acgaaggcac acagaaaaac gaccgtaccg gaaccggaac gctttccatt
      121 tttggtcatc agatgcgttt taacctgcaa gatggattcc cgctggtgac aactaaacgt
      181 tgccacctgc gttccatcat ccatgaactg ctgtggtttc tacagggcga cactaacatt
      241 gcttatctac acgaaaacaa tgtcaccatc tgggacgaat gggccgatga aaacggcgac
      301 ctcgggccag tgtatggtaa acagtggcgc gcctggccaa cgccagatgg tcgtcatatt
      361 gaccagatca ctacggtact gaaccagctg aaaaacgacc cggattcgcg ccgcattatt
      421 gtttcagcgt ggaacgtagg cgaactggat aaaatggcgc tggcaccgtg ccatgcattc
      481 ttccagttct atgtggcaga cggcaaactc tcttgccagc tttatcagcg ctcctgtgac
      541 gtcttcctcg gcctgccgtt caacattgcc agctacgcgt tattggtgca tatgatggcg
      601 cagcagtgcg atctggaagt gggtgatttt gtctggaccg gtggcgacac gcatctgtac
      661 agcaaccata tggatcaaac tcatctgcaa ttaagccgcg aaccgcgtcc gctgccgaag
      721 ttgattatca aacgtaaacc cgaatccatc ttcgactacc gtttcgaaga ctttgagatt
      781 gaaggctacg atccgcatcc gggcattaaa gcgccggtgg ctatctaagc ctagagacct
      841 actagtagcg gccgctgcag
//