##gff-version 3
##sequence-region BBa_J100132 1 860
BBa_J100132	.	stop_codon	826	828	.	+	0	ID=annotation2359045;Name=stop codon
BBa_J100132	.	engineered_region	1	22	.	+	0	ID=annotation2359037;Name=Prefix
BBa_J100132	.	start_codon	34	36	.	+	0	ID=annotation2359044;Name=start codon
BBa_J100132	.	non_covalent_binding_site	23	28	.	+	0	ID=annotation2359039;Name=BsaI cuts right
BBa_J100132	.	sequence_feature	829	832	.	+	0	ID=annotation2359043;Name=sticky end
BBa_J100132	.	sequence_feature	30	33	.	+	0	ID=annotation2359041;Name=sticky end
BBa_J100132	.	engineered_region	840	860	.	+	0	ID=annotation2359038;Name=Suffix
BBa_J100132	.	non_covalent_binding_site	834	839	.	+	0	ID=annotation2359042;Name=BsaI cuts left
BBa_J100132	.	CDS	35	828	.	+	0	ID=annotation2359046;Name=tyA gene
>BBa_J100132
gaattcgcggccgcttctagagggtctcagcgaatgaaacagtatttagaactgatgcaaaaagtgctcgacgaaggca
cacagaaaaacgaccgtaccggaaccggaacgctttccatttttggtcatcagatgcgttttaacctgcaagatggatt
cccgctggtgacaactaaacgttgccacctgcgttccatcatccatgaactgctgtggtttctacagggcgacactaac
attgcttatctacacgaaaacaatgtcaccatctgggacgaatgggccgatgaaaacggcgacctcgggccagtgtatg
gtaaacagtggcgcgcctggccaacgccagatggtcgtcatattgaccagatcactacggtactgaaccagctgaaaaa
cgacccggattcgcgccgcattattgtttcagcgtggaacgtaggcgaactggataaaatggcgctggcaccgtgccat
gcattcttccagttctatgtggcagacggcaaactctcttgccagctttatcagcgctcctgtgacgtcttcctcggcc
tgccgttcaacattgccagctacgcgttattggtgcatatgatggcgcagcagtgcgatctggaagtgggtgattttgt
ctggaccggtggcgacacgcatctgtacagcaaccatatggatcaaactcatctgcaattaagccgcgaaccgcgtccg
ctgccgaagttgattatcaaacgtaaacccgaatccatcttcgactaccgtttcgaagactttgagattgaaggctacg
atccgcatccgggcattaaagcgccggtggctatctaagcctagagacctactagtagcggccgctgcag