BBa_J100135 1 BBa_J100135 thyA Gene 2013-10-06T11:00:00Z 2015-08-31T04:08:23Z Promoter and C-Dog are from registry. ThyA is part BBa_J100132. This construct includes promoter P5 + C-Dog BD24 (bi-cistronic RBS) + thyA gene (BBa_J100132). This gene is used to complement the mutation in E. coli strain C600thyA-::Kan^R (Thompson et al., 2002). false false _578_ 0 14630 9 Not in stock false There are no internal EcoRI, XbaI, SpeI, PstI, or BsaI restriction sites. false Elizabeth Brunner annotation2369458 1 P5 (strong) range2369458 1 1 36 annotation2369460 1 C-Dog start range2369460 1 122 124 annotation2369462 1 thyA + 3 residues on N term. range2369462 1 122 922 annotation2369463 1 stop range2369463 1 923 925 annotation2369459 1 BD24 (strong C-Dog) range2369459 1 37 124 annotation2369461 1 thyA start range2369461 1 131 133 BBa_J100135_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatgcggcgaatgaaacagtatttagaactgatgcaaaaagtgctcgacgaaggcacacagaaaaacgaccgtaccggaaccggaacgctttccatttttggtcatcagatgcgttttaacctgcaagatggattcccgctggtgacaactaaacgttgccacctgcgttccatcatccatgaactgctgtggtttctacagggcgacactaacattgcttatctacacgaaaacaatgtcaccatctgggacgaatgggccgatgaaaacggcgacctcgggccagtgtatggtaaacagtggcgcgcctggccaacgccagatggtcgtcatattgaccagatcactacggtactgaaccagctgaaaaacgacccggattcgcgccgcattattgtttcagcgtggaacgtaggcgaactggataaaatggcgctggcaccgtgccatgcattcttccagttctatgtggcagacggcaaactctcttgccagctttatcagcgctcctgtgacgtcttcctcggcctgccgttcaacattgccagctacgcgttattggtgcatatgatggcgcagcagtgcgatctggaagtgggtgattttgtctggaccggtggcgacacgcatctgtacagcaaccatatggatcaaactcatctgcaattaagccgcgaaccgcgtccgctgccgaagttgattatcaaacgtaaacccgaatccatcttcgactaccgtttcgaagactttgagattgaaggctacgatccgcatccgggcattaaagcgccggtggctatctaagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z