BBa_J100136 1 BBa_J100136 T5+riboswitchD+thyA 2014-02-20T12:00:00Z 2015-08-31T04:08:23Z composite device from existing parts This part has a constitutive promoter (T5) followed by theophylline riboswitch D followed by thyA gene. This is used as a fitness module in thyA- cells that can produce theophylline. false false _578_ 0 201 61 Not in stock false We used golden gate assembly to put the thyA gene into T5+riboswitch into pSB1K8 plasmid. We used BsaI restriction enzyme. false Malcolm Campbell annotation2371835 1 start range2371835 1 160 162 annotation2371831 1 T5 constitutive range2371831 1 1 53 annotation2371832 1 riboswitch D (theophylline) range2371832 1 54 159 annotation2371833 1 thyA gene range2371833 1 160 954 annotation2371834 1 stop range2371834 1 952 954 BBa_J100136_sequence 1 aaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactagagatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgaaacagtatttagaactgatgcaaaaagtgctcgacgaaggcacacagaaaaacgaccgtaccggaaccggaacgctttccatttttggtcatcagatgcgttttaacctgcaagatggattcccgctggtgacaactaaacgttgccacctgcgttccatcatccatgaactgctgtggtttctacagggcgacactaacattgcttatctacacgaaaacaatgtcaccatctgggacgaatgggccgatgaaaacggcgacctcgggccagtgtatggtaaacagtggcgcgcctggccaacgccagatggtcgtcatattgaccagatcactacggtactgaaccagctgaaaaacgacccggattcgcgccgcattattgtttcagcgtggaacgtaggcgaactggataaaatggcgctggcaccgtgccatgcattcttccagttctatgtggcagacggcaaactctcttgccagctttatcagcgctcctgtgacgtcttcctcggcctgccgttcaacattgccagctacgcgttattggtgcatatgatggcgcagcagtgcgatctggaagtgggtgattttgtctggaccggtggcgacacgcatctgtacagcaaccatatggatcaaactcatctgcaattaagccgcgaaccgcgtccgctgccgaagttgattatcaaacgtaaacccgaatccatcttcgactaccgtttcgaagactttgagattgaaggctacgatccgcatccgggcattaaagcgccggtggctatctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z