BBa_J100166 1 BBa_J100166 P5 promoter + M6''-R10 Ammeline Riboswitch + RFP 2014-07-01T11:00:00Z 2015-08-31T04:08:23Z n/a This riboswitch sequence was built off of the original riboswitch sequence found in part J119335. It was designed to be a more effective riboswitch. The sequence contains three mutations from the original part. Two of which were designed (C to G at 77 and a C insert at 183) and one which occurred accidentally (T to C at 564). The accidental mutation caused no amino acid change (E. coli codon bias change from .34 to .4). false false _578_ 0 18885 9 Not in stock false n/a false Kathryn Smith annotation2379708 1 RFP range2379708 1 187 871 annotation2379706 1 P5 promoter range2379706 1 1 72 annotation2379707 1 M6''-R10 Ammeline Riboswitch range2379707 1 73 186 BBa_J100166_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaaggcttcatataatccgaatgatatggtttcggagcttccaccaagagccttaaactcttgattatgaagtctgtcgctttatccgaaattttataaagagaagactcatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggcaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z