BBa_J100170 1 BBa_J100170 Strong Promoter + M6"-R2 Ammeline Riboswitch + RFP 2014-07-09T11:00:00Z 2015-08-31T04:08:23Z n/a This construct is a basic biosensor for ammeline, based on part J119335. The P5 promoter drives the M6"-R2 riboswitch, which permits translation of the reporter gene (RFP) in the presence of ammeline. The M6"-R2 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). Mutation at position 430 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP CDS. false false _578_ 0 18888 9 Not in stock false n/a false Jonathan Lim annotation2380044 1 P5 range2380044 1 19 54 annotation2380115 1 A433G from original RFP range2380115 1 433 433 annotation2380045 1 M6"-R2 range2380045 1 73 191 annotation2380054 1 RFP range2380054 1 189 870 BBa_J100170_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaaggcttcatataatccgaatgatatggtttcggagcttccaccaagagccttaaactcttgattatgaagtcaacctgccttatccgaaattttataaggaggttgactcatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactgcctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z