BBa_J100172 1 BBa_J100172 Strong Promoter + M6"-R4 Ammeline Riboswitch + RFP 2014-07-09T11:00:00Z 2015-08-31T04:08:23Z Part J119335 but replaced the M6" riboswitch with the newly designed M6"-R4 riboswitch. This construct is a generic biosensor for ammeline, based on part J119335. The P5 promoter drives the M6"-R4 riboswitch, which permits translation of the reporter gene (RFP) in the presence of ammeline. The M6"-R4 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). Mutation at position 430 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP CDS. false false _578_ 0 18888 9 Not in stock false N/A false Jonathan Lim annotation2380133 1 RFP range2380133 1 188 869 annotation2380131 1 P5 range2380131 1 19 54 annotation2380130 1 A432G from original RFP range2380130 1 432 432 annotation2380132 1 M6"-R4 range2380132 1 73 190 BBa_J100172_sequence 1 ccgtatacacagaggaccttgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaaggcttcatataatccgaatgatatggtttcggagcttccaccaagagccttaaactcttgattatgaagtcaaccgccttatccgaaattttataaggaggttgactcatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactgcctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z