BBa_J100174 1 BBa_J100174 Strong Promoter + M6-R1 Ammeline Riboswitch + RFP 2014-07-24T11:00:00Z 2015-08-31T04:08:23Z J119332 This has a seven mutations from the original M6 riboswitch sequence found in part J119332. Five of these mutations are part of the intentional design of the riboswitch, in order for it to be more effective than the original M6 riboswitch. As with part J119332, base 432 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS. The two additional mutations are T20C in the promoter region and C852T in the RFP CDS. false false _578_ 0 18860 9 Not in stock false N/A false Hannah Itell annotation2380293 1 A to G Change range2380293 1 432 432 annotation2380294 1 T to C Change range2380294 1 20 20 annotation2380291 1 M6-R1 Ammeline Riboswitch range2380291 1 73 187 annotation2380295 1 C to T Change range2380295 1 852 852 annotation2380290 1 P5 range2380290 1 18 55 annotation2380292 1 RFP range2380292 1 185 869 BBa_J100174_sequence 1 ccgtatacacagaggacctcgacaattaatcatccggctcgtaatttatgtggatgggtcttgaaccaggagtcaaggcttcatataatcctaatgatatggtttgggagcttccaccaagagccttaaactcttgattatgaagtctgcctgctttatccgaaattttataaagaggagactcatgaaggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactgcctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcacttcaccggtgcttaataagcct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z