BBa_J100183
1
JEAN
P R/tetO
2014-09-10T11:00:00Z
2015-08-31T04:08:23Z
The genomic sequence comes from rhizobial phage 16-3.
This is a hybrid promoter that was engineered from a P R promote from rhizobial phage 16-3, along with its companion hybrid P R/tetO. It was engineered to be inducible by p-isopropyl benzoate (cumate)(Q) or anhydrotetracycline (aTc). P R/cmtO was observed to respond to a range of 0.1 to 5 ??g/ml (0.6 to 30 ??M) of Q and the P R/tetO promoter from 0.1 to 25 ng/ml (0.2 nM to 50 nM) aTc. This promoter was designed for use in Methylobacterium extorquens.
http://www.biomedcentral.com/1756-0500/6/183
"A novel pair of inducible expression vectors for use in Methylobacterium extorquens."
false
false
_578_
0
23984
9
Not in stock
false
We designed the sticky ends to be inducible with BsaI.
false
Erin Xu, Nick Elder, Julia Preziosi, Abby Hunt
annotation2382878
1
-10
range2382878
1
42
47
annotation2382879
1
-35
range2382879
1
22
27
BBa_J100183_sequence
1
cgacaaggcaaacaatggtacttgacgactcatcacaacaattgtagttgtagattgtaagcgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z