LOCUS       BBa_J100198             1000 bp    DNA     linear       22-Nov-2024
DEFINITION  J100198 contains the fitness gene tester construct (J100199) with 
            thymidylate synthase as the fitnes
ACCESSION   BBa_J100198
VERSION     BBa_J100198.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Ecor1
     misc_feature    7..169
                     /label=T5 promoter+ theophylline riboswitch 
     CDS             170..954
                     /label=thymidylate synthase 
     misc_feature    955..986
                     /label=LVA degradation tag
     misc_feature    987..989
                     /label=stop
     misc_feature    990..995
                     /label=Pst1
ORIGIN
        1 gaattcgaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc
       61 aattgtgagc ggataacaat tactagagat acgactcact ataggtaccg gtgataccag
      121 catcgtcttg atgcccttgg cagcaccctg ctaaggtaac aacaagatga aacagtattt
      181 agaactgatg caaaaagtgc tcgacgaagg cacacagaaa aacgaccgta ccggaaccgg
      241 aacgctttcc atttttggtc atcagatgcg ttttaacctg caagatggat tcccgctggt
      301 gacaactaaa cgttgccacc tgcgttccat catccatgaa ctgctgtggt ttctacaggg
      361 cgacactaac attgcttatc tacacgaaaa caatgtcacc atctgggacg aatgggccga
      421 tgaaaacggc gacctcgggc cagtgtatgg taaacagtgg cgcgcctggc caacgccaga
      481 tggtcgtcat attgaccaga tcactacggt actgaaccag ctgaaaaacg acccggattc
      541 gcgccgcatt attgtttcag cgtggaacgt aggcgaactg gataaaatgg cgctggcacc
      601 gtgccatgca ttcttccagt tctatgtggc agacggcaaa ctctcttgcc agctttatca
      661 gcgctcctgt gacgtcttcc tcggcctgcc gttcaacatt gccagctacg cgttattggt
      721 gcatatgatg gcgcagcagt gcgatctgga agtgggtgat tttgtctgga ccggtggcga
      781 cacgcatctg tacagcaacc atatggatca aactcatctg caattaagcc gcgaaccgcg
      841 tccgctgccg aagttgatta tcaaacgtaa acccgaatcc atcttcgact accgtttcga
      901 agactttgag attgaaggct acgatccgca tccgggcatt aaagcgccgg tggctatcgc
      961 tgcaaacgac gaaaactacg ctttagtagc ttaactgcag
//