BBa_J100198 1 BBa_J100198 J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes 2014-12-01T12:00:00Z 2015-08-31T04:08:23Z N/A Into the fitness gene tester construct, J100199, J100198 contains the fitness gene thymidylate synthase. J100198 contains the a theophylline riboswitch downstream of a constitutive promoter. the construct is built to be used in thymine auxotrophs that necessitate thymine for growth and division. Upon successful binding of theophylline on the riboswitch, thymidylate synthase should be expressed for the auxotrophs. false false _578_ 0 18881 9 Not in stock true N/A false Telavive Taye annotation2430265 1 thymidylate synthase range2430265 1 170 954 annotation2430268 1 Pst1 range2430268 1 990 995 annotation2430264 1 T5 promoter+ theophylline riboswitch range2430264 1 7 169 annotation2430266 1 LVA degradation tag range2430266 1 955 986 annotation2430267 1 stop range2430267 1 987 989 annotation2430263 1 Ecor1 range2430263 1 1 6 BBa_J100198_sequence 1 gaattcgaaatcataaaaaatttatttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactagagatacgactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgaaacagtatttagaactgatgcaaaaagtgctcgacgaaggcacacagaaaaacgaccgtaccggaaccggaacgctttccatttttggtcatcagatgcgttttaacctgcaagatggattcccgctggtgacaactaaacgttgccacctgcgttccatcatccatgaactgctgtggtttctacagggcgacactaacattgcttatctacacgaaaacaatgtcaccatctgggacgaatgggccgatgaaaacggcgacctcgggccagtgtatggtaaacagtggcgcgcctggccaacgccagatggtcgtcatattgaccagatcactacggtactgaaccagctgaaaaacgacccggattcgcgccgcattattgtttcagcgtggaacgtaggcgaactggataaaatggcgctggcaccgtgccatgcattcttccagttctatgtggcagacggcaaactctcttgccagctttatcagcgctcctgtgacgtcttcctcggcctgccgttcaacattgccagctacgcgttattggtgcatatgatggcgcagcagtgcgatctggaagtgggtgattttgtctggaccggtggcgacacgcatctgtacagcaaccatatggatcaaactcatctgcaattaagccgcgaaccgcgtccgctgccgaagttgattatcaaacgtaaacccgaatccatcttcgactaccgtttcgaagactttgagattgaaggctacgatccgcatccgggcattaaagcgccggtggctatcgctgcaaacgacgaaaactacgctttagtagcttaactgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z