LOCUS       BBa_J100199              245 bp    DNA     linear       13-May-2024
DEFINITION  Fitness Gene Tester + LVA degradation tag
ACCESSION   BBa_J100199
VERSION     BBa_J100199.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcorI site
     promoter        7..58
                     /label=T5 Promoter
     misc_feature    59..164
                     /label=Theophylline RiboswitchD
     misc_feature    165..167
                     /label=start codon
     misc_feature    171..176
                     /label=BsaI site
     misc_feature    177..197
                     /label=spacer
     misc_feature    197..202
                     /label=BsaI site
     misc_feature    204..236
                     /label=LVA d??gradation tag
     misc_feature    237..239
                     /label=Stop codon
     misc_feature    240..245
                     /label=PstI site
ORIGIN
        1 gaattcgaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc
       61 aattgtgagc ggataacaat tactagagat acgactcact ataggtaccg gtgataccag
      121 catcgtcttg atgcccttgg cagcaccctg ctaaggtaac aacaagatgt gagaccgcca
      181 gctttacggt ctttatggtc tctgctgcaa acgacgaaaa ctacgcttta gtagcttaac
      241 tgcag
//