LOCUS       BBa_J100200             2188 bp    DNA     linear       16-Dec-2024
DEFINITION  tClone tetA v2
ACCESSION   BBa_J100200
VERSION     BBa_J100200.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 (strong) promoter facing right
     misc_feature    42..47
                     /label=BsaI cuts left
     misc_feature    37..40
                     /label=sticky end
     CDS             55..774
                     /label=GFP (backwards)
     RBS             781..795
                     /label=B0030 (backwards)
     promoter        803..848
                     /label=P2 (strong; backwards)
     stem_loop       849..898
                     /label=transcriptional terminator L3S1P53
     misc_feature    899..904
                     /label=BsaI cuts right
     misc_feature    906..909
                     /label=sticky end
     RBS             910..995
                     /label=BD18, C-Dog
     CDS             995..2188
                     /label=tetA antibiotic resistance
     misc_feature    2183..2188
                     /label=double stop
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccact agagttatta
       61 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      121 catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      301 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
      361 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
      481 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
      541 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
      661 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
      721 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac gcatctagta
      781 tttctcctct ttaattacta gatccacaca ttataggtac aaaaagatgc gaagtcaata
      841 ctctttttcc aattattgaa ggcctcccaa atcggggggc cttttttatt gataacaagg
      901 tctctgcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca attttcgtac
      961 tgaaacatct taatcatgcg acggagcgtt tctaatgaaa tctaacaatg cgctcatcgt
     1021 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
     1081 gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct
     1141 agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
     1201 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
     1261 catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac
     1321 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     1381 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     1441 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     1501 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     1561 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     1621 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     1681 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     1741 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggccc
     1801 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     1861 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     1921 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     1981 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     2041 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     2101 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     2161 atggagccgg gccacctcga cctaataa
//