##gff-version 3
##sequence-region BBa_J100200 1 2188
BBa_J100200	.	stem_loop	849	898	.	+	0	ID=annotation2418024;Name=transcriptional terminator L3S1P53
BBa_J100200	.	non_covalent_binding_site	42	47	.	+	0	ID=annotation2418019;Name=BsaI cuts left
BBa_J100200	.	sequence_feature	906	909	.	+	0	ID=annotation2418026;Name=sticky end
BBa_J100200	.	stop_codon	2183	2188	.	+	0	ID=annotation2418029;Name=double stop
BBa_J100200	.	promoter	803	848	.	+	0	ID=annotation2418023;Name=P2 (strong; backwards)
BBa_J100200	.	ribosome_entry_site	781	795	.	+	0	ID=annotation2418022;Name=B0030 (backwards)
BBa_J100200	.	ribosome_entry_site	910	995	.	+	0	ID=annotation2418027;Name=BD18, C-Dog
BBa_J100200	.	promoter	1	36	.	+	0	ID=annotation2418018;Name=P5 (strong) promoter facing right
BBa_J100200	.	CDS	55	774	.	+	0	ID=annotation2418021;Name=GFP (backwards)
BBa_J100200	.	CDS	995	2188	.	+	0	ID=annotation2418028;Name=tetA antibiotic resistance
BBa_J100200	.	non_covalent_binding_site	899	904	.	+	0	ID=annotation2418025;Name=BsaI cuts right
BBa_J100200	.	sequence_feature	37	40	.	+	0	ID=annotation2418020;Name=sticky end
>BBa_J100200
ttgacaattaatcatccggctcgtaatttatgtggacgactgagaccactagagttattatttgtatagttcatccatg
ccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaaggg
cagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtc
tgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgcc
atgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaatac
cttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatcttt
gaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctggg
tatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgc
aaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaac
atcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctct
ttaattactagatccacacattataggtacaaaaagatgcgaagtcaatactctttttccaattattgaaggcctccca
aatcggggggccttttttattgataacaaggtctctgcgggggcccaagttcacttaaaaaggagatcaacaatgaaag
caattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgaaatctaacaatgcgctcatcgtcatcctc
ggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccatt
ccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcgg
agcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatg
gcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctg
gcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtggg
tatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctc
aacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagag
ccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcat
gcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatc
ggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcg
gcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctg
gatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcag
gtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctga
tcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgt
ctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataa