BBa_J100200 1 BBa_J100200 tClone tetA v2 2014-10-12T11:00:00Z 2015-08-31T04:08:23Z This was designed in the Campbell and Eckdahl labs and is related to plasmid J119137 (pClone Red). This device is used to test new transcriptional termination riboswitches. The default color of colonies would be green and they would be tetracycline sensitive. Users employ golden gate assembly (GGA) to remove the cassette flanked by BsaI sites and clone into this location their transcriptional termination riboswitch. In the presence or absence of ligand, the contract would become tetracycline resistant, or not, depending if the riboswitch was an on or off switch. false false _578_ 0 201 61 Not in stock false Need to screen for internal BsaI sites. Flanked by BioBrick endings and having this synthesized as two G blocks so that we can clone this faster and cheaper than as an entire gene. false Malcolm Campbell annotation2418024 1 transcriptional terminator L3S1P53 range2418024 1 849 898 annotation2418019 1 BsaI cuts left range2418019 1 42 47 annotation2418026 1 sticky end range2418026 1 906 909 annotation2418029 1 double stop range2418029 1 2183 2188 annotation2418023 1 P2 (strong; backwards) range2418023 1 803 848 annotation2418022 1 B0030 (backwards) range2418022 1 781 795 annotation2418027 1 BD18, C-Dog range2418027 1 910 995 annotation2418018 1 P5 (strong) promoter facing right range2418018 1 1 36 annotation2418021 1 GFP (backwards) range2418021 1 55 774 annotation2418028 1 tetA antibiotic resistance range2418028 1 995 2188 annotation2418025 1 BsaI cuts right range2418025 1 899 904 annotation2418020 1 sticky end range2418020 1 37 40 BBa_J100200_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgactgagaccactagagttattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcatctagtatttctcctctttaattactagatccacacattataggtacaaaaagatgcgaagtcaatactctttttccaattattgaaggcctcccaaatcggggggccttttttattgataacaaggtctctgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z