BBa_J100201
1
BBa_J100201
RBS (BD24) + GFP
2014-10-23T11:00:00Z
2015-08-31T04:08:24Z
We assembled the part from preexisting parts which were isolated and cloned together in one plasmid.
This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. There is also an EcoRI recognition site preceding the RBS.
false
false
_578_
0
201
61
Not in stock
false
There are no BsaI sites.
false
Malcolm Campbell
annotation2429302
1
T-->C
range2429302
1
140
140
annotation2429298
1
EcoRI site
range2429298
1
1
6
annotation2429299
1
RBS BD24
range2429299
1
9
94
annotation2429301
1
A-->G
range2429301
1
111
111
annotation2429300
1
superfolding GFP
range2429300
1
94
810
annotation2429610
1
Stop
range2429610
1
808
810
BBa_J100201_sequence
1
gaattcgcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatgcgtaaaggcgaagagctgtttaccggtgttgttccgattctggctgaactggatggtgatgttaatggccacaaattttcagttcgtggcgaaggtgaaggtgatgcaaccaatggtaaactgaccctgaaatttatctgtaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgttttgcacgttatccggatcatatgaaacagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatctcctttaaagatgatggcacctataaaacccgtgccgaagttaaatttgaaggcgataccctggtgaatcgtattgagctgaaaggcatcgatttcaaagaggatggtaatatcctgggccataaactggaatataacttcaatagccacaatgtgtatatcaccgcagacaaacagaaaaacggcattaaagccaactttaagattcgccataatgtggaagatggtagcgtgcagctggcagatcattatcagcagaataccccgattggtgatggtccggttctgctgccggataatcactatctgagcacccagagcgttctgagcaaagatccgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccatggtatggatgaactgtacaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z