BBa_J100201 1 BBa_J100201 RBS (BD24) + GFP 2014-10-23T11:00:00Z 2015-08-31T04:08:24Z We assembled the part from preexisting parts which were isolated and cloned together in one plasmid. This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. There is also an EcoRI recognition site preceding the RBS. false false _578_ 0 201 61 Not in stock false There are no BsaI sites. false Malcolm Campbell annotation2429302 1 T-->C range2429302 1 140 140 annotation2429298 1 EcoRI site range2429298 1 1 6 annotation2429299 1 RBS BD24 range2429299 1 9 94 annotation2429301 1 A-->G range2429301 1 111 111 annotation2429300 1 superfolding GFP range2429300 1 94 810 annotation2429610 1 Stop range2429610 1 808 810 BBa_J100201_sequence 1 gaattcgcgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatgcgtaaaggcgaagagctgtttaccggtgttgttccgattctggctgaactggatggtgatgttaatggccacaaattttcagttcgtggcgaaggtgaaggtgatgcaaccaatggtaaactgaccctgaaatttatctgtaccaccggcaaactgccggttccgtggccgaccctggttaccaccctgacctatggtgttcagtgttttgcacgttatccggatcatatgaaacagcacgattttttcaaaagcgcaatgccggaaggttatgttcaagaacgtaccatctcctttaaagatgatggcacctataaaacccgtgccgaagttaaatttgaaggcgataccctggtgaatcgtattgagctgaaaggcatcgatttcaaagaggatggtaatatcctgggccataaactggaatataacttcaatagccacaatgtgtatatcaccgcagacaaacagaaaaacggcattaaagccaactttaagattcgccataatgtggaagatggtagcgtgcagctggcagatcattatcagcagaataccccgattggtgatggtccggttctgctgccggataatcactatctgagcacccagagcgttctgagcaaagatccgaatgaaaaacgtgatcacatggtgctgctggaatttgttaccgcagcaggtattacccatggtatggatgaactgtacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z