BBa_J100202 1 BBa_J100202 Intermediate for IndClone Red 2014-11-21T12:00:00Z 2015-08-31T04:08:24Z E. coli-based genes We are building indClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid. false false _578_ 0 201 61 Not in stock false No BsaI sites so we will be able to later use Golden Gate Assembly. false Malcolm Campbell annotation2430247 1 RBS - BD21 (J119027) range2430247 1 39 126 annotation2430248 1 Red fluorescent protein & BD18 (strong RBS) E1010_BD18 range2430248 1 124 804 annotation2430246 1 partial Promoter R0082 range2430246 1 1 38 annotation2430249 1 PstI recognition site range2430249 1 805 810 BBa_J100202_sequence 1 cgtgttggattattctgcatttttggggagaatggactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgagggatggtttctaatggcaagcagcgaagatgtgattaaagaatttatgcgcttcaaggtgcgtatggaaggtagcgttaatggtcatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatattccggattacctgaaattaagctttccggaaggttttaaatgggaacgcgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcttacaggatggtgagtttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgtctgaagctgaaagacggtggtcattatgatgcagaagttaaaaccacctacatggccaaaaaaccggttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagtaccggtgcctaataactgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z