LOCUS       BBa_J100205             2414 bp    DNA     linear       22-Nov-2024
DEFINITION  repClone Red
ACCESSION   BBa_J100205
VERSION     BBa_J100205.1  
FEATURES             Location/Qualifiers
     CDS             1..639
                     /label=tetR Repressor C0041
     RBS             640..727
                     /label=RBS BD24
     promoter        728..763
                     /label=P5 Promoter (for TetR)
     CDS             764..1483
                     /label=GFP 
     RBS             1490..1501
                     /label=RBS B0034
     misc_feature    1517..1522
                     /label=BsaI site
     promoter        1523..1568
                     /label=P2 Promoter
     misc_feature    1577..1637
                     /label=Terminator L3S2P21
     misc_feature    1638..1643
                     /label=BsaI site
     RBS             1649..1736
                     /label=RBS BD18
     CDS             1734..2414
                     /label=RFP
ORIGIN
        1 cggccgctac tagtattatt agctaccgct ttcacatttc agttgttttt ccagtccgca
       61 aataatcagt tctaaaccaa acagaaaggc aggttctgca ccctgatgat caaacagttc
      121 aatggcctga cgcagcagcg gaggcatgct atcggtggtc ggggtttcgc gttcttcttt
      181 tgcaacctga tgttcttgat cttccagaac acaacccagg gtaaaatgac caactgcgct
      241 cagtgcatac agtgcgtttt ccagactaaa accttgctga cacagaaatg ccagctgatt
      301 ttccagggtt tcatactgtt tttcggtcgg acgggtgccc agatgaactt ttgcaccatc
      361 acgatggctc agcagggcac aacgaaagct ttttgcatta ttacgcagaa aatcctgcca
      421 gctttcacct tccagcggac aaaaatgggt atgatgacga tccagcattt caattgccag
      481 tgcatccagc agtgcacgtt tgtttttaac atgccaatac agggtcggct gttcaacacc
      541 cagtttctgt gccagtttac gggtggtcag accttcaata ccaacttcat tcagcagttc
      601 cagtgcgcta ttaatcactt tgcttttatc cagacggctc attagaaacc gtccatcgca
      661 tgattaagat gtttcagtac gaaaattgct ttcattgttg atctcctttt taagtgaact
      721 tgggccctcc acataaatta cgagccggat gattaattgt caattattat ttgtatagtt
      781 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtgatctc
      841 tctcttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      901 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      961 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
     1021 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatat
     1081 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
     1141 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat acagttcttt
     1201 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
     1261 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
     1321 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     1381 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcgccatct aattcaacaa
     1441 gaattgggac aactccagtg aaaagttctt ctcctttacg cacctagtat ttctcctctt
     1501 taattactag acgacagaga cctccacaca ttataggtac aaaaagatgc gaagtcaata
     1561 ctctttttgg ccaattctcg gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc
     1621 ttttttcgtt ttggtccggt ctctgcgggg gcccaagttc acttaaaaag gagatcaaca
     1681 atgaaagcaa ttttcgtact gaaacatctt aatcatgcga cggagcgttt ctaatggctt
     1741 cctccgaaga tgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
     1801 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     1861 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
     1921 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
     1981 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagatggtg
     2041 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
     2101 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
     2161 gggaagcttc caccgaacgt atgtacccgg aagatggtgc tctgaaaggt gaaatcaaaa
     2221 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
     2281 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
     2341 cccacaacga agattacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     2401 ccggtgctta ataa
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