BBa_J100205 1 BBa_J100205 repClone Red 2014-12-07T12:00:00Z 2016-10-10T11:40:19Z The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010). We designed repClone Red to test the function of repressors. The tetR repressor is present but inactive because pTet is not included. Addition of pTet will prevent translation of GFP. Addition of tetracycline to the environment (or anhydrotetracycline) will prevent inhibition by tetR. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules. false false _578_ 18858 18858 9 Not in stock false The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix. false Monica Prudencio annotation2433031 1 Terminator L3S2P21 range2433031 1 1577 1637 annotation2433025 1 RBS BD24 range2433025 1 640 727 annotation2433029 1 BsaI site range2433029 1 1517 1522 annotation2433026 1 P5 Promoter (for TetR) range2433026 1 728 763 annotation2433030 1 P2 Promoter range2433030 1 1523 1568 annotation2433033 1 RBS BD18 range2433033 1 1649 1736 annotation2433028 1 RBS B0034 range2433028 1 1490 1501 annotation2433027 1 GFP range2433027 1 764 1483 annotation2433024 1 tetR Repressor C0041 range2433024 1 1 639 annotation2433032 1 BsaI site range2433032 1 1638 1643 annotation2433034 1 RFP range2433034 1 1734 2414 BBa_J100205_sequence 1 cggccgctactagtattattagctaccgctttcacatttcagttgtttttccagtccgcaaataatcagttctaaaccaaacagaaaggcaggttctgcaccctgatgatcaaacagttcaatggcctgacgcagcagcggaggcatgctatcggtggtcggggtttcgcgttcttcttttgcaacctgatgttcttgatcttccagaacacaacccagggtaaaatgaccaactgcgctcagtgcatacagtgcgttttccagactaaaaccttgctgacacagaaatgccagctgattttccagggtttcatactgtttttcggtcggacgggtgcccagatgaacttttgcaccatcacgatggctcagcagggcacaacgaaagctttttgcattattacgcagaaaatcctgccagctttcaccttccagcggacaaaaatgggtatgatgacgatccagcatttcaattgccagtgcatccagcagtgcacgtttgtttttaacatgccaatacagggtcggctgttcaacacccagtttctgtgccagtttacgggtggtcagaccttcaataccaacttcattcagcagttccagtgcgctattaatcactttgcttttatccagacggctcattagaaaccgtccatcgcatgattaagatgtttcagtacgaaaattgctttcattgttgatctcctttttaagtgaacttgggccctccacataaattacgagccggatgattaattgtcaattattatttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtgatctctctcttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatatttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatacagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcgccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttacgcacctagtatttctcctctttaattactagacgacagagacctccacacattataggtacaaaaagatgcgaagtcaatactctttttggccaattctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccggtctctgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z