BBa_J100207
1
BBa_J100207
tClone Red RS3
2015-06-01T11:00:00Z
2015-08-31T04:08:24Z
J119361; Wachsmuth et. al
We took out everything between the BsaI sites in J119361. We then inserted the RS3 riboswitch, the sequence of which was found in De novo design of a synthetic riboswitch that regulates transcription termination (Wachsmuth et. al).
false
false
_578_
0
23980
9
Not in stock
false
There is a BsaI site in the pUC IDT Amp plasmid that may confound results by cutting at a different site.
false
Camille Johnson
annotation2432579
1
RS3 Riboswitch
range2432579
1
41
120
annotation2432582
1
BD18 C dog RBS
range2432582
1
124
215
annotation2432577
1
P5 Promoter
range2432577
1
1
34
annotation2432586
1
RFP
range2432586
1
216
887
BBa_J100207_sequence
1
ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcatttacatactcggtaaactgaagtgctgccattttttttgcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z